| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,076,301 | A→C | H22P (CAC→CCC) | sbcB → | exodeoxyribonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,076,301 | 0 | A | C | 96.9% | 109.9 / ‑3.2 | 32 | H22P (CAC→CCC) | sbcB | exodeoxyribonuclease I |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (16/15); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTAC > NZ_CP009273/2076166‑2076436 | tgGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc < 2:761103/138‑1 (MQ=255)tgGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc < 2:729981/138‑1 (MQ=255)tgGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc < 1:473603/138‑1 (MQ=255) aGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGa < 2:338238/139‑1 (MQ=255) aGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGa < 1:594894/139‑1 (MQ=255) tGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGtt > 2:611639/1‑139 (MQ=255) tGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGtt > 1:441678/1‑139 (MQ=255) tGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGtt > 2:687354/1‑139 (MQ=255) tGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGtt < 2:704702/139‑1 (MQ=255) atatTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCg < 1:659393/139‑1 (MQ=255) agcagAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc < 1:688712/91‑1 (MQ=255) agcagAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc > 2:688712/1‑91 (MQ=255) cagAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGaa > 2:220659/1‑139 (MQ=255) atgatgAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGa < 1:454399/77‑1 (MQ=255) atgatgAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGa > 2:454399/1‑77 (MQ=255) atgatgAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCg < 1:397211/139‑1 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGcc > 1:626123/1‑76 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGcc < 2:626123/76‑1 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAAc < 1:367559/130‑1 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAAc > 2:367559/1‑130 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCt > 2:568709/1‑139 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCt > 2:382461/1‑139 (MQ=255) aatgaCGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCt > 2:473904/1‑139 (MQ=255) aGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGc > 2:588367/1‑137 (MQ=255) aGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCaa < 1:170534/139‑1 (MQ=255) ccTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc < 1:537988/39‑1 (MQ=255) ccTTTTTGTTTCACGATTACGAAACCTTTGGCACGcccc > 2:537988/1‑39 (MQ=255) ccTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCtgatg < 2:265664/138‑1 (MQ=255) tttGTTTCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTAt > 2:623096/1‑139 (MQ=255) tCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGcc < 1:83405/44‑1 (MQ=255) tCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGcc > 2:83405/1‑44 (MQ=255) tCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCg < 1:103162/95‑1 (MQ=255) tCACGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCg > 2:103162/1‑95 (MQ=255) cGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGcaca > 2:399632/1‑48 (MQ=255) cGATTACGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGcaca < 1:399632/48‑1 (MQ=255) aCGAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCccagccag < 1:687354/138‑1 (MQ=255) gAAACCTTTGGCACGCCCCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAg < 2:441678/139‑1 (MQ=255) tGGCACGCCCCACGCGTTAGATCGCCCTGCACAGTTCGCa > 2:577676/1‑40 (MQ=255) tGGCACGCCCCACGCGTTAGATCGCCCTGCACAGTTCGCa < 1:577676/40‑1 (MQ=255) cgcccCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTAc < 1:623096/139‑1 (MQ=255) cgcccCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTAc < 1:473904/139‑1 (MQ=255) cgcccCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTAc < 1:382461/139‑1 (MQ=255) | TGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTAC > NZ_CP009273/2076166‑2076436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |