Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,121,860	A→G	N170N (AAT→AAC)	wcaF ←	colanic acid biosynthesis acetyltransferase WcaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,121,860	0	A	G	100.0%	111.9 / NA	32	N170N (AAT→AAC)	wcaF	colanic acid biosynthesis acetyltransferase WcaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

TCTTGTCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATC  >  NZ_CP009273/2121755‑2121992                                                                                                          |                                                                                                                                     tCTTGTCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGAt                                                                                                     >  1:492721/1‑139 (MQ=255) tCTTGTCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGAt                                                                                                     >  2:12525/1‑139 (MQ=255) tCTTGTCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGAt                                                                                                     >  1:741118/1‑139 (MQ=255)      tCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTaa                                                                                                 >  1:135862/1‑138 (MQ=255)             acacCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACt                                                                                          <  1:12525/138‑1 (MQ=255)                   gTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGt                                                                                   <  2:515647/139‑1 (MQ=255)                   gTGATGAGAGCGACTGTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGt                                                                                   <  2:540180/139‑1 (MQ=255)                         agagCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGc                                                                                 >  1:216364/1‑135 (MQ=255)                         agagCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGc                                                                                 <  2:216364/135‑1 (MQ=255)                          gagCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCa                                                                            <  2:257186/139‑1 (MQ=255)                                     gACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGcc                                                                  <  1:653964/138‑1 (MQ=255)                                                ttCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATtgt                                                      <  2:135862/139‑1 (MQ=255)                                                  ccTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATtgtg                                                     <  2:75220/138‑1 (MQ=255)                                                    tctGGATTTTTGAATTTATGCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACg                                                  >  2:765309/1‑139 (MQ=255)                                                     ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTg                                                                                         <  1:606692/99‑1 (MQ=255)                                                     ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTg                                                                                         >  2:606692/1‑99 (MQ=255)                                                     ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCAcc                                                                              >  2:477959/1‑110 (MQ=255)                                                     ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCAcc                                                                              <  1:477959/110‑1 (MQ=255)                                                     ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGc                                                 >  1:578608/1‑139 (MQ=255)                                                        tATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGcc                                                <  1:568507/137‑1 (MQ=255)                                                        tATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTg                                              >  1:111334/1‑139 (MQ=255)                                                        tATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTg                                              <  2:151316/139‑1 (MQ=255)                                                                  tttATTCAGTTTCAACGCGTTCGCGTTTCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGcaaca                                      >  2:440632/1‑137 (MQ=255)                                                                                     ttCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGccagccag                 <  1:541809/139‑1 (MQ=255)                                                                                        gcgTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCAt              <  1:690335/139‑1 (MQ=255)                                                                                             tCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGcaacaa                                     >  2:342660/1‑111 (MQ=255)                                                                                             tCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGcaacaa                                     <  1:342660/111‑1 (MQ=255)                                                                                                 cacTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCa     <  2:578608/139‑1 (MQ=255)                                                                                                   cTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAAt   <  2:111334/139‑1 (MQ=255)                                                                                                    tGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAAt   >  2:318238/1‑138 (MQ=255)                                                                                                    tGCGGGGGTCCCCCGTCAATCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACTCACGCCGGTGCCGTCGCCGTTTGTGACGCCTGGGGTATCGCAGACATCGGTTGCCAGCCAGCATTTACCGCCaagc  >  2:733049/1‑137 (MQ=255)                                                                                                    tGCGGGGATCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAACAACAATGCTTCGTGCACCCACGACGGTGCCGTCGCAGATTGTGACGCCTGGGGCAACAAAGACAGCGGTTGCCAGCCAGCATGTCTCTCCAATc  >  2:148837/1‑139 (MQ=255)                                                                                                          |                                                                                                                                     TCTTGTCCGGTTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATC  >  NZ_CP009273/2121755‑2121992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑