Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,141,824	T→C	S224P (TCA→CCA)	yegD →	molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,141,824	0	T	C	100.0%	71.8 / NA	21	S224P (TCA→CCA)	yegD	molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/16);  total (5/16)

CGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGCTGG  >  NZ_CP009273/2141703‑2141959                                                                                                                          |                                                                                                                                        cGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATgggg                                                                                                                        <  2:95900/139‑1 (MQ=255)              gTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGtt                                                                                                           <  2:259290/139‑1 (MQ=255)              gTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTCCATTGCTGCTGATGGGGGCGCAGTgggggt                                                                                                           >  1:543435/1‑135 (MQ=255)                               gCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGAAGACTGACTGTCCATTGCTGCTGGTGGGGGCGCGGGGGGGGTTGGGTCTCGGTCggg                                                                                            >  2:71492/1‑135 (MQ=255)                                             ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctgctg                                                                              <  1:356496/137‑1 (MQ=255)                                             ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctgctg                                                                              <  2:112660/137‑1 (MQ=255)                                             ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTggg                                                                            <  1:117558/139‑1 (MQ=255)                                                  gATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTcaca                                                                       <  2:121523/139‑1 (MQ=255)                                                  gATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTcac                                                                        <  1:133524/138‑1 (MQ=255)                                                  gATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGGACGGCCGTCCCTTGCTGCTGGTGGGGGGGCCGGGGGGGTTGCGTCTCCGTCGGGGAGCCCGCCTGCTggggcg                                                                         >  2:125463/1‑134 (MQ=255)                                                    ttACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGt                                                                     <  1:71492/139‑1 (MQ=255)                                                                         agaaAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAAc                                                <  2:201925/139‑1 (MQ=255)                                                                            aaaaCGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGAt                                             <  1:62287/139‑1 (MQ=255)                                                                              aaCGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCt                                           <  1:600378/139‑1 (MQ=255)                                                                                     gCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTCCCTTTCTGCTGGTGGGGGCGCAGTGGCGGTCGCGTCTCGGTCGTGAAGCCAGCCTGCTGGGGCACAGTGGTTGCCGTATTGGCGGGGAAGATCTTGgtatt                                    >  1:266316/1‑138 (MQ=255)                                                                                             tggATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGc                            <  1:530874/139‑1 (MQ=255)                                                                                                               cGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGc           <  2:602660/138‑1 (MQ=255)                                                                                                                 actgactgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCAc        <  2:418851/139‑1 (MQ=255)                                                                                                                     actgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActg      <  2:543435/137‑1 (MQ=255)                                                                                                                     aatgTCCATTGCTGCTGAGGGGGCCGGAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCGGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActg      <  2:135190/135‑1 (MQ=255)                                                                                                                       tgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGCTgg  >  2:254900/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                        CGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGCTGG  >  NZ_CP009273/2141703‑2141959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑