Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,633,239	(T)8→7	intergenic (‑159/‑232)	uspB ← / → uspA	universal stress protein UspB/universal stress protein UspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,633,232	0	T	.	100.0%	161.7 / NA	37	intergenic (‑152/‑239)	uspB/uspA	universal stress protein UspB/universal stress protein UspA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/12);  total (25/12)

TTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTA  >  NZ_CP009273/3633110‑3633364                                                                                                                           |                                                                                                                                     ttCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGtt                                                                                                                     <  1:534024/139‑1 (MQ=255)  tCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGtt                                                                                                                     >  2:534024/1‑138 (MQ=255)     aCCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAAcc                                                                                                                 <  1:155351/139‑1 (MQ=255)        ccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACt                                                                                                              <  2:135960/139‑1 (MQ=255)        ccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACt                                                                                                              <  1:454022/139‑1 (MQ=255)                               cTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAAt                                                                                       >  2:520445/1‑139 (MQ=255)                                 gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAAttt                                                                                     >  1:114791/1‑139 (MQ=255)                                          ttAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCt                                                                             >  2:47287/1‑138 (MQ=255)                                                               aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                        >  2:558875/1‑138 (MQ=255)                                                               aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                        >  2:109756/1‑138 (MQ=255)                                                               aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                        >  1:67103/1‑138 (MQ=255)                                                               aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                        >  2:616458/1‑138 (MQ=255)                                                                              aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGAACATCCGGGt                                        <  2:114791/139‑1 (MQ=255)                                                                                   cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCta                                   <  1:399492/139‑1 (MQ=255)                                                                                             gCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTAtaaggtaagg                         <  1:616458/139‑1 (MQ=255)                                                                                             gCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTAtaaggtaag                          >  1:587319/1‑138 (MQ=255)                                                                                                      atagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCtt                 >  1:73516/1‑138 (MQ=255)                                                                                                       tagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTAt                                                                                                            >  1:119648/1‑46 (MQ=255)                                                                                                       tagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTAt                                                                                                            <  2:119648/46‑1 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                        <  2:178865/113‑1 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                        >  1:178865/1‑113 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                       >  2:44320/1‑130 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                       <  1:44320/130‑1 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTaa               >  2:91821/1‑138 (MQ=255)                                                                                                        agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTaa               >  1:534352/1‑138 (MQ=255)                                                                                                          tgtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAacac            >  1:82860/1‑139 (MQ=255)                                                                                                          tgtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAacac            >  1:75856/1‑139 (MQ=255)                                                                                                           gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt           >  1:194426/1‑139 (MQ=255)                                                                                                              ggAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGa         <  2:194426/138‑1 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCTCTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGAc                                                     <  2:195632/88‑1 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGAc                                                     >  1:195632/1‑88 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  2:280125/1‑139 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  1:489204/1‑139 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  1:448059/1‑139 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  1:350871/1‑139 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  2:609572/1‑139 (MQ=255)                                                                                                                    cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAAAGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa  >  2:504286/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                     TTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTA  >  NZ_CP009273/3633110‑3633364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑