Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,058,644	Δ1 bp	coding (483/1404 nt)	yihO ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,058,644	0	A	.	100.0%	151.9 / NA	35	coding (483/1404 nt)	yihO	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/16);  total (19/16)

GCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGG  >  NZ_CP009273/4058512‑4058768                                                                                                                                     |                                                                                                                             gCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCAccc                                                                                                                        <  2:73843/138‑1 (MQ=255)    caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGc                                                                                                                    >  2:608409/1‑139 (MQ=255)    caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGc                                                                                                                    >  2:353067/1‑139 (MQ=255)    caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAAAGCCACGGCACACATCAATAGCCCTATGGATGC‑CCACCCTGGc                                                                                                                    >  2:546189/1‑139 (MQ=255)    caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGCTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGc                                                                                                                    >  2:300415/1‑139 (MQ=255)       catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                                  >  1:446851/1‑138 (MQ=255)         tcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTaa                                                                                                               <  2:348730/139‑1 (MQ=255)                           cAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcgagcgc                                                                                             >  2:469688/1‑139 (MQ=255)                                gagaACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCAtt                                                                                        <  2:201386/139‑1 (MQ=255)                                   aaCATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCgg                                                                                     >  1:47777/1‑139 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                         >  1:37857/1‑139 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                         <  2:446851/139‑1 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                         >  1:38504/1‑139 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                         >  1:72380/1‑139 (MQ=255)                                                                  ttGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCa                                                      <  1:4268/139‑1 (MQ=255)                                                                     aGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATAc                                                   >  2:45557/1‑139 (MQ=255)                                                                      gTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCgg                                                                   <  1:306316/122‑1 (MQ=255)                                                                      gTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCgg                                                                   >  2:306316/1‑122 (MQ=255)                                                                         aGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                                <  1:437953/138‑1 (MQ=255)                                                                                  cGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                      <  2:38504/139‑1 (MQ=255)                                                                                     cTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGAc                                   >  1:344451/1‑139 (MQ=255)                                                                                            ggAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGa                            <  1:406667/139‑1 (MQ=255)                                                                                                  aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                              <  1:499158/115‑1 (MQ=255)                                                                                                  aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                              >  2:499158/1‑115 (MQ=255)                                                                                                    cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                    <  1:434709/139‑1 (MQ=255)                                                                                                    cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                    <  2:636961/139‑1 (MQ=255)                                                                                                    cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                    <  2:571044/139‑1 (MQ=255)                                                                                                        aCGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                              >  2:282054/1‑109 (MQ=255)                                                                                                        aCGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                              <  1:282054/109‑1 (MQ=255)                                                                                                                     aTAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGcgcg   <  1:353067/139‑1 (MQ=255)                                                                                                                      tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCgg  >  2:543452/1‑139 (MQ=255)                                                                                                                      tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCgg  >  1:465374/1‑139 (MQ=255)                                                                                                                      tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCgg  >  1:530806/1‑139 (MQ=255)                                                                                                                            tATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatc                                       >  2:615949/1‑96 (MQ=255)                                                                                                                            tATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatc                                       <  1:615949/96‑1 (MQ=255)                                                                                                                                     |                                                                                                                             GCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGG  >  NZ_CP009273/4058512‑4058768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑