Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,464,092	(C)6→5	coding (152/594 nt)	bdcR →	TetR/AcrR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,464,087	0	C	.	100.0%	124.5 / NA	29	coding (147/594 nt)	bdcR	TetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/15);  total (14/15)

TCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTG  >  NZ_CP009273/4463959‑4464215                                                                                                                                 |                                                                                                                                 tctcGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGATGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCct                                                                                                                       >  1:66974/1‑139 (MQ=255)      cgTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTAcg                                                                                                                  <  2:505426/139‑1 (MQ=255)      cgTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTAcg                                                                                                                  <  1:295302/139‑1 (MQ=255)                 gtcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTgg                                                                                                        >  2:308247/1‑138 (MQ=255)                 gtcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTgg                                                                                                        >  1:473220/1‑138 (MQ=255)                  tcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACTCGGCTTTTgg                                                                                                        >  2:113298/1‑137 (MQ=255)                   cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                     >  2:508719/1‑139 (MQ=255)                   cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                     >  1:446245/1‑139 (MQ=255)                    ccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAg                                                                                                     <  1:308247/138‑1 (MQ=255)                            ttCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCct                                                                                                                       <  2:413803/112‑1 (MQ=255)                            ttCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCct                                                                                                                       >  1:413803/1‑112 (MQ=255)                             tCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTggg                                                                                           >  1:321946/1‑139 (MQ=255)                                   cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt                                                                                      <  2:505340/138‑1 (MQ=255)                                   cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGATGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt                                                                                      <  2:66974/138‑1 (MQ=255)                                      aGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGc                                                                                  >  1:257250/1‑139 (MQ=255)                                           gCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAAcccccgagc                                                                                                                          >  1:444824/1‑91 (MQ=255)                                           gCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAAcccccgagc                                                                                                                          <  2:444824/95‑5 (MQ=255)                                            cAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTa                                                                            <  1:141320/139‑1 (MQ=255)                                                ctctGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTc                                                                         >  1:602999/1‑138 (MQ=255)                                                     cGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTg                                                                                             <  2:156604/113‑1 (MQ=255)                                                     cGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTg                                                                                             >  1:156604/1‑113 (MQ=255)                                                     cGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGaa                                                                   >  2:592334/1‑139 (MQ=255)                                                          aaaGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt                                                              <  1:167515/139‑1 (MQ=255)                                                                     tCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGaa                                                    <  1:113298/138‑1 (MQ=255)                                                                               ggTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCt                                         <  1:592334/139‑1 (MQ=255)                                                                                                     cTGAAGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTgatgat                   >  2:182544/1‑139 (MQ=255)                                                                                                         aGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTc               <  2:189027/139‑1 (MQ=255)                                                                                                         aGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTc               <  2:513640/139‑1 (MQ=255)                                                                                                         aGTTACTGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTc               <  1:508719/139‑1 (MQ=255)                                                                                                               tGATTATCTTGGTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAg         >  2:366936/1‑139 (MQ=255)                                                                                                                         ggTATTAA‑CCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTg  <  2:446245/136‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 TCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTG  >  NZ_CP009273/4463959‑4464215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑