Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,148,0820AG41.2% 19.8 / 38.9 34S196G (AGC→GGC) mdtAmultidrug efflux RND transporter subunit MdtA
Reads supporting (aligned to +/- strand):  ref base A (12/8);  new base G (8/6);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCT  >  NZ_CP009273/2147946‑2148215
                                                                                                                                        |                                                                                                                                     
ttATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGc                                                                                                                                     >  2:446922/1‑139 (MQ=255)
             gCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCAc                                                                                                                        >  2:236565/1‑139 (MQ=255)
                                              gATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTg                                                                                       <  1:236565/139‑1 (MQ=255)
                                                  cccAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGt                                                                                   <  1:313584/139‑1 (MQ=255)
                                                     aaCAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTgg                                                                                >  1:517293/1‑139 (MQ=255)
                                                             cTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaaa                                                                        >  1:114467/1‑139 (MQ=255)
                                                                   aGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCa                                                                  <  2:517293/139‑1 (MQ=255)
                                                                        aaCCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAgtggt                                                             >  1:614422/1‑139 (MQ=255)
                                                                               ggCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATacca                                                      >  2:543300/1‑139 (MQ=255)
                                                                                            cTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                                         <  1:446922/139‑1 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGc                                                                                                                                     >  1:349988/1‑45 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGc                                                                                                                                     <  2:349988/45‑1 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAg                                                                                              <  1:60048/84‑1 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAg                                                                                              >  2:60048/1‑84 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                                       >  1:356473/1‑139 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTcgcg                                                                                                         <  2:161169/73‑1 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTcgcg                                                                                                         >  1:161169/1‑73 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                                       >  1:550370/1‑139 (MQ=255)
                                                                                              gatgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                                       >  2:444180/1‑139 (MQ=255)
                                                                                               atgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                                      <  1:543300/139‑1 (MQ=255)
                                                                                                tgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                                      >  2:556231/1‑138 (MQ=255)
                                                                                                tgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                                      >  1:551758/1‑138 (MQ=255)
                                                                                                      aagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACAGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTTGTGATCCCCCa                                >  2:140145/1‑138 (MQ=255)
                                                                                                        gcGTTGCCAGCGCGCAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGAc                             <  2:172590/139‑1 (MQ=255)
                                                                                                        gcGTGGCAAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGAc                             <  2:594346/139‑1 (MQ=255)
                                                                                                         cGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACg                            >  2:293311/1‑139 (MQ=255)
                                                                                                                  cgcgcAGCTGCAACTCGACTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATc                   >  1:294033/1‑139 (MQ=255)
                                                                                                                      cAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAtt               <  2:614422/139‑1 (MQ=255)
                                                                                                                       aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt              <  1:293311/139‑1 (MQ=255)
                                                                                                                       aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt              <  2:60659/139‑1 (MQ=255)
                                                                                                                                   aCTGGGGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCt  >  1:457592/1‑139 (MQ=255)
                                                                                                                                   aCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt              <  2:574113/127‑1 (MQ=255)
                                                                                                                                   aCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt              >  1:574113/1‑127 (MQ=255)
                                                                                                                                   aCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCt  >  1:180290/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCT  >  NZ_CP009273/2147946‑2148215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑