Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,284,1710AG42.9% 23.7 / 49.0 43T667A (ACG→GCG) yraJfimbrial biogenesis outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base A (15/9);  new base G (8/10);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACCATAACAGTAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAG  >  NZ_CP009273/3284037‑3284308
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acCATAACAGTAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGct                                                                                                                                       <  2:521079/139‑1 (MQ=255)
          tAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGc                                                                                                                                  <  2:336525/134‑1 (MQ=255)
          tAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGc                                                                                                                                  <  2:249175/134‑1 (MQ=255)
          tAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGc                                                                                                                                  >  1:336525/1‑134 (MQ=255)
          tAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGc                                                                                                                                  >  1:249175/1‑134 (MQ=255)
           aGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATc                                                                                                                            >  2:346732/1‑139 (MQ=255)
            gCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCt                                                                                                                           >  1:257016/1‑139 (MQ=255)
                 aaCGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGc                                                                                                                       >  1:116630/1‑138 (MQ=255)
                  aCGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCCCGCAATATCTGGGCaa                                                                                                                     >  2:568160/1‑139 (MQ=255)
                           aCCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTg                                                                                                             <  2:101813/138‑1 (MQ=255)
                                       aTGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGTCGATCTCGCCATATCTGGGCAACGCTTTTGCGCTTATCGTTGc                                                                                                >  1:265120/1‑139 (MQ=255)
                                              cTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTAt                                                                                                      <  2:621481/126‑1 (MQ=255)
                                              cTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTAt                                                                                                      >  1:621481/1‑126 (MQ=255)
                                                  aGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCAt                                                                                     <  2:426740/139‑1 (MQ=255)
                                                         gATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGt                                                                              >  2:197053/1‑139 (MQ=255)
                                                         gATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGt                                                                              >  2:379288/1‑139 (MQ=255)
                                                           tACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGa                                                                            <  1:197053/139‑1 (MQ=255)
                                                              aGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGa                                                                         >  1:622323/1‑139 (MQ=255)
                                                              aGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGa                                                                         >  2:403478/1‑139 (MQ=255)
                                                                cTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa                                                                       >  2:499731/1‑139 (MQ=255)
                                                                        aTGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTa                                                               >  1:377415/1‑139 (MQ=255)
                                                                            cagcagCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCg                                                           <  2:423875/139‑1 (MQ=255)
                                                                             agcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                                                                                          <  1:339336/107‑1 (MQ=255)
                                                                             agcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACgg                                                                                          >  2:339336/1‑107 (MQ=255)
                                                                               cagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCggg                                                         >  1:277628/1‑138 (MQ=255)
                                                                               cagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCggg                                                         >  2:248486/1‑138 (MQ=255)
                                                                                 gcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGAt                                                      <  2:377415/139‑1 (MQ=255)
                                                                                  ccAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGAtt                                                     <  2:88575/139‑1 (MQ=255)
                                                                                     aTACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGc                                                   >  1:597568/1‑138 (MQ=255)
                                                                                         aaTTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGACGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCg                                              <  2:423071/139‑1 (MQ=255)
                                                                                                gCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCttt                                       <  1:346732/139‑1 (MQ=255)
                                                                                                gCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCttt                                       <  1:526617/139‑1 (MQ=255)
                                                                                                              gTGGTTATCCATCCTCATGGCGTGGCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTgg                         <  2:189330/139‑1 (MQ=255)
                                                                                                                ggTTATCCATCCTCATGGCGGGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACAGATCCTTTTGGCTATGCAGTGGtt                       >  2:320150/1‑139 (MQ=255)
                                                                                                                    atccatccTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCt                    <  1:248486/138‑1 (MQ=255)
                                                                                                                    atccatccTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCt                    <  1:136266/138‑1 (MQ=255)
                                                                                                                          ccTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaa                                                                    >  1:446542/1‑84 (MQ=255)
                                                                                                                          ccTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaa                                                                    <  2:446542/84‑1 (MQ=255)
                                                                                                                          ccTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTca             >  1:294377/1‑139 (MQ=255)
                                                                                                                            tCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTgg                                    <  2:227959/114‑1 (MQ=255)
                                                                                                                            tCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTgg                                    >  1:227959/1‑114 (MQ=255)
                                                                                                                                    tGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTAt     >  2:195214/1‑137 (MQ=255)
                                                                                                                                     gACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAg  >  2:84580/1‑139 (MQ=255)
                                                                                                                                      aCGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAg  >  2:603563/1‑138 (MQ=255)
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ACCATAACAGTAGCCTGAACGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAG  >  NZ_CP009273/3284037‑3284308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑