Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,392,3630TC50.0% 2.7 / 36.1 28Q325Q (CAA→CAG) mreCrod shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base T (7/7);  new base C (6/8);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGC  >  NZ_CP009273/3392227‑3392495
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cTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTAGCCGGCtg                                                                                                                                    >  2:625724/1‑139 (MQ=255)
          ttGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAg                                                                                                                            <  2:622684/137‑1 (MQ=255)
          ttGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAg                                                                                                                            <  1:13535/137‑1 (MQ=255)
          ttGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAg                                                                                                                            <  1:285546/137‑1 (MQ=255)
             cccTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGc                                                                                                                       <  2:507838/139‑1 (MQ=255)
                                        aGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGt                                                                                            <  2:378116/139‑1 (MQ=255)
                                           accacTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAAc                                                                                         >  1:241571/1‑139 (MQ=255)
                                               cTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGt                                                                                                  <  1:316351/126‑1 (MQ=255)
                                               cTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGt                                                                                                  >  2:316351/1‑126 (MQ=255)
                                                                ccTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGc                                                                    <  1:157633/139‑1 (MQ=255)
                                                                 cTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg                                                                   <  2:241571/139‑1 (MQ=255)
                                                                     gtgGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAg                                                                <  1:624824/138‑1 (MQ=255)
                                                                        gagaGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCaa                                                            <  2:618872/139‑1 (MQ=255)
                                                                          gagCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATa                                                          >  2:547049/1‑139 (MQ=255)
                                                                              gATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTg                                                      >  1:155136/1‑139 (MQ=255)
                                                                                   gcagcaGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGca                                                 <  1:122369/139‑1 (MQ=255)
                                                                                     agcagGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGcatc                                               <  2:455459/139‑1 (MQ=255)
                                                                                          gCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGt                                                                                            <  1:281398/89‑1 (MQ=255)
                                                                                          gCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGt                                                                                            >  2:281398/1‑89 (MQ=255)
                                                                                          gCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTg                                          >  2:114676/1‑139 (MQ=255)
                                                                                                    cgcAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGt                                >  2:77487/1‑139 (MQ=255)
                                                                                                    cgcAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGt                                >  1:329746/1‑139 (MQ=255)
                                                                                                      gagcagGCGCAGTAGGTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGGGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCCTCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTc                              <  1:116774/138‑1 (MQ=255)
                                                                                                        gcagGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCAt                            >  2:360494/1‑139 (MQ=255)
                                                                                                        gcagGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACg                                 >  2:502897/1‑134 (MQ=255)
                                                                                                        gcagGCGCAGTAGCTGCATTTCCTGTCGCCGGCTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACg                                 <  1:502897/134‑1 (MQ=255)
                                                                                                                        cATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCAc            >  1:273460/1‑139 (MQ=255)
                                                                                                                                  cGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGc  >  2:102467/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGC  >  NZ_CP009273/3392227‑3392495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑