| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,852,866 | 0 | A | C | 57.1% | 5.1 / 17.2 | 14 | V204G (GTG→GGG) | yidK | solute:sodium symporter family transporter |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGGCAAGGGATCGGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGC > NZ_CP009273/3852746‑3852985 | aTCGGCAAGGGATCGGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATaa > 1:38416/1‑139 (MQ=255) tCGGAAGGGGATCGGGCGGGACCCCGATTGATTTTAATTTCTCGGCGTGACCGGTGGTGAGTTGCTCAACCCCCTGCAAAAAGCTGCCCTCG‑CCCACCGCGATTAGCCAAAATCCCGCCCCCAACACCCCGCCGATAAc < 2:45278/139‑1 (MQ=255) gcGGCAGGGGATCGGTGGGCCCACCGATTGATTTTAATTTCCCGGCGTGAAGGGGGGTGAGTTGCTCAATGCCCTGCAAAAAGCTGCCCTTG‑CCCACCGCGATTAGGCCAAACCCCGGCCCCATCAACCCGCCGATAAc < 1:348884/138‑1 (MQ=255) ccGGCAAGGGATCGGTCGGGCCACCGTTTGATTTTATTTTCTCGGCGTGACGGGTGGTGAGTTGCTCAATGCCCTGCAAAAAGCTCCCCTTC‑CCCATCGCAATTAGCCAAAATCCCGCCCCCACCACCCCGCCGATAAc < 1:424181/138‑1 (MQ=255) gggtgcggtcggGCCCCCGTTTGAGTTTATTTCCGCGGCGTAAGGGGTGGGGAGTTGCTCAATCCCCTGAATAAAGCGGCCTTTC‑CCCATCGCAATTAGGCCAAATCCCGGCCCCACAACCCCGCCGATAACCAGCCCa < 1:225618/134‑1 (MQ=255) ggttcggtcggCCCACCGATTGATTTTAATTTCCCGGCGTGAACGGTGGTGAGTTGCCCAATCCCCTGCAAAAAGCTCCCCTTG‑CCCTTCGCGATTAGGCAAAATACCGCCCCCATCACCCCGCCGATAACCAGCCCaa < 1:353433/136‑1 (MQ=255) gtcggGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGt > 2:225618/1‑139 (MQ=255) gccggCCCACCTATTGATTTTAATTCCTCGGCGTGACCGGTGGTGAGTTGCTCAATCCCCTGCATAAAGCTGCCCTTC‑CCCACCGCGATTAGGCCAAATCCCGCCCCCATCACCCCGCCGATAACCAGCCCAATACCGt < 1:446097/137‑1 (MQ=255) ggcccACCGATTGATTTTATTTTCCCGGCGTGACCGGTGGTGAGTTCCTCAATCCCCTGCATAAACCTCCCCTTC‑CCCATCGCGATTAGGCCAAATCCCGCCCCCACCAACCCGCCGATAACCAGCCCAATACCGTTGa < 1:568744/136‑1 (MQ=255) gCCACCGATTGAGGTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATg > 2:476480/1‑139 (MQ=255) aGTTTAATTTCTCGGAGTCAGCGGTGGTAATTTCCTCAATGCCCAGCA‑CAAGCCGCCCCTGCCCCAAGGAGATTGGAAAAAACACCGACCCCACAAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCa < 1:583663/138‑1 (MQ=255) tGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCg > 1:278954/1‑139 (MQ=255) cccTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGc > 1:76594/1‑138 (MQ=255) ttAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGc > 2:56970/1‑137 (MQ=255) | ATCGGCAAGGGATCGGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTG‑CCCATCGCGATTAGGCCAAATACCGGCACCATCAACCCGCCGATAACCAGCCCAATACCGTTGATGGAGTCCGCCACTGCCATTGCGCGCAGTCCGCCGATCACCGCATACAAAATTCCCGCCAGACCAAGCAAAATTACCAGTAGC > NZ_CP009273/3852746‑3852985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |