Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	10,865	A→C	G164G (GGT→GGG)	msyB ←	acidic protein MsyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	10,865	0	A	C	100.0%	61.9 / NA	18	G164G (GGT→GGG)	msyB	acidic protein MsyB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/7);  total (11/7)

CTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTG  >  NZ_CP009273/10732‑10999                                                                                                                                      |                                                                                                                                          cTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCttt                                                                                                                                      >  1:174101/1‑139 (MQ=255)                gTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGt                                                                                                                        <  1:76133/138‑1 (MQ=255)                 tGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCt                                                                                                                      <  1:193137/139‑1 (MQ=255)                 tGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCt                                                                                                                      >  2:193978/1‑139 (MQ=255)                    cGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCttt                                                                                                                    >  2:608566/1‑138 (MQ=255)                      cACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTtcat                                                                                                                 >  1:525244/1‑139 (MQ=255)                                                        caAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                            <  2:596532/139‑1 (MQ=255)                                                                gCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATc                                                                    >  1:103579/1‑139 (MQ=255)                                                                       tACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACcgcg                                                             <  1:623316/139‑1 (MQ=255)                                                                                tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAAc                                                    >  1:577565/1‑139 (MQ=255)                                                                                tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAAc                                                    >  2:336440/1‑139 (MQ=255)                                                                                 gctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGa                                                       <  1:608566/138‑1 (MQ=255)                                                                                 gctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACt                                                   >  1:92980/1‑139 (MQ=255)                                                                                   tgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                                                 >  1:539566/1‑139 (MQ=255)                                                                                     cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                            <  2:281681/110‑1 (MQ=255)                                                                                     cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                            >  1:281681/1‑110 (MQ=255)                                                                                            gCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                                >  1:429673/1‑99 (MQ=255)                                                                                            gCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                                <  2:429673/99‑1 (MQ=255)                                                                                                                                  acacccCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTg  >  1:35469/1‑139 (MQ=255)                                                                                                                                  acacccCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCCCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTg  >  2:72608/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                          CTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCAT‑‑‑CAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTG  >  NZ_CP009273/10732‑10999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑