Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,253,834	A→G	I352I (ATT→ATC)	fruA ←	PTS fructose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,253,834	0	A	G	100.0%	147.0 / NA	39	I352I (ATT→ATC)	fruA	PTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/23);  total (16/23)

CCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGC  >  NZ_CP009273/2253716‑2253946                                                                                                                       |                                                                                                                 ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                              <  1:88519/139‑1 (MQ=255) ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                              <  1:496020/139‑1 (MQ=255)    cATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcgc                                                                                           >  1:597784/1‑139 (MQ=255)      ttCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCt                                                                                         >  1:248180/1‑139 (MQ=255)                       cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGt                                                                        >  2:515458/1‑139 (MQ=255)                         gCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcgc                                                                                           >  1:99914/1‑118 (MQ=255)                         gCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcgc                                                                                           <  2:99914/118‑1 (MQ=255)                                gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                            >  2:70437/1‑110 (MQ=255)                                gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                            <  1:70437/110‑1 (MQ=255)                                 tGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCg                                                              <  1:619559/139‑1 (MQ=255)                                       aGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTg                                                         <  1:444032/138‑1 (MQ=255)                                               gagaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACtt                                                 <  2:600831/138‑1 (MQ=255)                                                  aaTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCa                                             <  1:577543/139‑1 (MQ=255)                                                       cAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTa                                        <  2:62342/139‑1 (MQ=255)                                                         gCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAAc                                      <  2:463396/139‑1 (MQ=255)                                                                        aTCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCg                       <  2:466805/139‑1 (MQ=255)                                                                         tCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTa                                                            <  1:185801/101‑1 (MQ=255)                                                                         tCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTa                                                            >  2:185801/1‑101 (MQ=255)                                                                         tCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGc                      <  2:486423/139‑1 (MQ=255)                                                                          cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                           >  1:307262/1‑85 (MQ=255)                                                                          cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                           <  2:307262/85‑1 (MQ=255)                                                                                 atcatcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATac              <  1:437586/139‑1 (MQ=255)                                                                                     tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                            <  1:341868/57‑1 (MQ=255)                                                                                     tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                            >  2:341868/1‑57 (MQ=255)                                                                                      cGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAAtt                                                                 >  2:478667/1‑83 (MQ=255)                                                                                      cGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAAtt                                                                 <  1:478667/83‑1 (MQ=255)                                                                                       gCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCaa        >  2:184797/1‑139 (MQ=255)                                                                                        ccAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAAt       <  2:248180/139‑1 (MQ=255)                                                                                        ccAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAAt       <  1:515458/139‑1 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGttt                                                                      <  1:545643/68‑1 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGttt                                                                      >  2:545643/1‑68 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAAtt                                                                 <  2:114432/73‑1 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAAtt                                                                 >  1:114432/1‑73 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCg                       >  1:638901/1‑115 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCg                       <  2:638901/115‑1 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc  >  2:1453/1‑136 (MQ=255)                                                                                                accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc  >  1:346284/1‑136 (MQ=255)                                                                                                          tGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGc                      <  2:322325/106‑1 (MQ=255)                                                                                                          tGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGc                      >  1:322325/1‑106 (MQ=255)                                                                                                                       |                                                                                                                 CCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGC  >  NZ_CP009273/2253716‑2253946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑