Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,483,956	A→G	S492P (TCA→CCA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,483,956	0	A	G	100.0%	95.7 / NA	28	S492P (TCA→CCA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

GTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAG  >  NZ_CP009273/2483823‑2484084                                                                                                                                      |                                                                                                                                 gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGgtggtggtg                                                                                                                             >  2:310713/1‑139 (MQ=255) gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGgtggtggtg                                                                                                                             >  2:593785/1‑139 (MQ=255) gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGgtggtggtg                                                                                                                             >  1:520948/1‑139 (MQ=255)              aCTGAATACCGGTTGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  2:140776/139‑1 (MQ=255)              aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  1:600293/139‑1 (MQ=255)              aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                >  1:376525/1‑139 (MQ=255)              aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  2:34070/139‑1 (MQ=255)              aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  1:593785/139‑1 (MQ=255)                       cGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAac                                                                                                       <  1:398060/139‑1 (MQ=255)                         gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAgcg                                                                                                                     >  2:213763/1‑123 (MQ=255)                         gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAgcg                                                                                                                     <  1:213763/123‑1 (MQ=255)                         gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAacac                                                                                                     >  1:579424/1‑139 (MQ=255)                            gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGt                                                                                                  >  2:273094/1‑139 (MQ=255)                                aaGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGtctc                                                                                               <  1:273094/138‑1 (MQ=255)                                    aTGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTg                                                                                          >  2:333869/1‑139 (MQ=255)                                      gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCa                                                                                                                  >  2:326426/1‑113 (MQ=255)                                      gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCa                                                                                                                  <  1:326426/113‑1 (MQ=255)                                      gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTg                                                                                          >  2:94453/1‑137 (MQ=255)                                       aCGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTg                                                                                          >  1:480431/1‑136 (MQ=255)                                                          gtgACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTtggtggtgg                                                                                                                               <  1:65059/80‑1 (MQ=255)                                                          gtgACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTtggtggtgg                                                                                                                               >  2:65059/1‑80 (MQ=255)                                                               gTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAAc                                                                >  2:633739/1‑138 (MQ=255)                                                                ttATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGa                                                              <  1:333869/139‑1 (MQ=255)                                                                         aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                     <  1:633739/139‑1 (MQ=255)                                                                          cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCgg                                                    >  2:382002/1‑139 (MQ=255)                                                                               cGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGt                                               >  2:432378/1‑139 (MQ=255)                                                                                                                  gTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACt             >  2:375008/1‑138 (MQ=255)                                                                                                                   tGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg            >  2:251457/1‑138 (MQ=255)                                                                                                                   tGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg            >  1:383594/1‑138 (MQ=255)                                                                                                                     cAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa          <  1:251457/138‑1 (MQ=255)                                                                                                                           aTCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCaaaa   <  2:520948/139‑1 (MQ=255)                                                                                                                             cctttGGTTGTGGTGCACCAGCGCCAATGAGATCTACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg  <  2:143606/135‑1 (MQ=255)                                                                                                                             ccGTTGGTGGTGGTGCACCAGCGCCACTGAGACCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg  >  1:559992/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                 GTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAG  >  NZ_CP009273/2483823‑2484084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑