Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,511,537	A→G	noncoding (59/76 nt)	BW25113_RS12500 ←	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,511,537	0	A	G	100.0%	102.5 / NA	29	noncoding (59/76 nt)	BW25113_RS12500	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

GATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAC  >  NZ_CP009273/2511403‑2511665                                                                                                                                       |                                                                                                                                 gATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGa                                                                                                                              <  2:235906/139‑1 (MQ=255)      ttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCg                                                                                                                          <  2:457854/138‑1 (MQ=255)          tCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGAc                                                                                                                     <  2:82865/139‑1 (MQ=255)               tgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCtt                                                                                                                <  1:636637/139‑1 (MQ=255)                       tCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGcatgcca                                                                                                        >  1:447379/1‑139 (MQ=255)                                   aCTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctct                                                                                            >  1:110210/1‑139 (MQ=255)                                   aCTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctct                                                                                            <  2:46571/139‑1 (MQ=255)                                       aCCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTcc                                                                                          >  1:260152/1‑137 (MQ=255)                                            gACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGAc                                                                                                                     >  1:523206/1‑105 (MQ=255)                                            gACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGAc                                                                                                                     <  2:523206/105‑1 (MQ=255)                                                 aGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                                               >  2:59242/1‑138 (MQ=255)                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                                         >  1:147222/1‑138 (MQ=255)                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                                         >  1:163743/1‑138 (MQ=255)                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                                         >  1:358468/1‑138 (MQ=255)                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                                         <  1:410991/138‑1 (MQ=255)                                                            cAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGa                                                                    <  2:447379/138‑1 (MQ=255)                                                             aaaaGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATg                                                                  <  1:59242/139‑1 (MQ=255)                                                               aaGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCg                                                                >  1:123387/1‑139 (MQ=255)                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTg                                                                                    >  2:535594/1‑117 (MQ=255)                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTg                                                                                    <  1:535594/117‑1 (MQ=255)                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt                                                              >  2:38437/1‑139 (MQ=255)                                                                                           cccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaa                                     >  2:97375/1‑138 (MQ=255)                                                                                                      gCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg                         <  2:358468/139‑1 (MQ=255)                                                                                                      gCGAGGGTTGGAAAGTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg                         <  2:159986/139‑1 (MQ=255)                                                                                                         aGGGTTGGAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCt                       >  2:57721/1‑138 (MQ=255)                                                                                                               ggAAATTTGGTGGAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCgg                 >  1:193196/1‑138 (MQ=255)                                                                                                                      tggtggAGCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAc         >  2:222514/1‑139 (MQ=255)                                                                                                                             gCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAc  <  2:263262/139‑1 (MQ=255)                                                                                                                             gCTAAGCGGGGTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAc  <  2:147222/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 GATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAC  >  NZ_CP009273/2511403‑2511665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑