Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,547,539	A→G	S335P (TCC→CCC)	eutR ←	HTH‑type transcriptional regulator EutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,547,539	0	A	G	100.0%	95.0 / NA	27	S335P (TCC→CCC)	eutR	HTH‑type transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

AATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGG  >  NZ_CP009273/2547407‑2547655                                                                                                                                     |                                                                                                                     aaTATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAg                                                                                                                <  2:48793/139‑1 (MQ=255)         tATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTgg                                                                                                         >  1:163330/1‑138 (MQ=255)           tCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTgg                                                                                                         >  1:173001/1‑136 (MQ=255)           tCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTgg                                                                                                         <  2:173001/136‑1 (MQ=255)             aGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAAt                                                                                                     <  2:517883/138‑1 (MQ=255)               ccAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCg                                                                                                  >  1:27322/1‑139 (MQ=255)               ccAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCg                                                                                                  >  1:368940/1‑139 (MQ=255)                       tGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAAt                                                                                          <  2:27322/139‑1 (MQ=255)                                       gcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAg                                                                           >  2:224573/1‑138 (MQ=255)                                          ttAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGATGCTGGTAATCCGAGGCAAATTGCCCCAGAAGCCACAAtg                                                                       >  2:617541/1‑138 (MQ=255)                                                          aGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcggc                                                       <  2:163330/139‑1 (MQ=255)                                                                  gATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTa                                               <  2:518906/139‑1 (MQ=255)                                                                       ggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgc                                                                                                               <  2:119079/70‑1 (MQ=255)                                                                       ggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgc                                                                                                               >  1:119079/1‑70 (MQ=255)                                                                           tAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa                                                                   <  1:91747/110‑1 (MQ=255)                                                                           tAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa                                                                   >  2:91747/1‑110 (MQ=255)                                                                                           aCTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACt                      >  2:260172/1‑139 (MQ=255)                                                                                                 gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATccc                                                                     >  1:461741/1‑86 (MQ=255)                                                                                                 gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATccc                                                                     <  2:461741/86‑1 (MQ=255)                                                                                                      cGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcg            <  1:374409/138‑1 (MQ=255)                                                                                                       gCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgcg          >  2:193578/1‑139 (MQ=255)                                                                                                       gCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgcg          >  1:399378/1‑139 (MQ=255)                                                                                                          gATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCgcggcg       >  1:98644/1‑139 (MQ=255)                                                                                                              cAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACg   <  2:98644/139‑1 (MQ=255)                                                                                                               aGCGTCAGTGACGGCTTCTCGGGAAACATCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCCGTTCGCGGCGTACgg  >  1:638413/1‑139 (MQ=255)                                                                                                                     aGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCAc                           >  2:216920/1‑108 (MQ=255)                                                                                                                     aGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCAc                           <  1:216920/108‑1 (MQ=255)                                                                                                                                     |                                                                                                                     AATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGG  >  NZ_CP009273/2547407‑2547655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑