Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,561,055	A→G	S138P (TCC→CCC)	eutH ←	ethanolamine utilization protein EutH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,561,055	0	A	G	100.0%	81.7 / NA	22	S138P (TCC→CCC)	eutH	ethanolamine utilization protein EutH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

CACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAG  >  NZ_CP009273/2560924‑2561172                                                                                                                                    |                                                                                                                      cacaCCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAAcaca                                                                                                                <  1:374537/139‑1 (MQ=255)                aGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCcat                                                                                                  >  1:228757/1‑138 (MQ=255)                              caGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAAt                                                                                   <  1:367757/139‑1 (MQ=255)                                   aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                              >  1:196104/1‑139 (MQ=255)                                   aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                              <  2:230685/139‑1 (MQ=255)                                   aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                              <  2:169184/139‑1 (MQ=255)                                   aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                              <  2:614330/139‑1 (MQ=255)                                      cAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCa                                                                            <  1:392022/138‑1 (MQ=255)                                             tCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCg                                                                                        <  2:215439/119‑1 (MQ=255)                                             tCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCg                                                                                        >  1:215439/1‑119 (MQ=255)                                              cGGAATGGTCACAATGCCCGCCAGCACGCCGATCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGc                                                                   >  2:172053/1‑139 (MQ=255)                                               ggAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGc                                                                   <  1:323952/138‑1 (MQ=255)                                                gAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCa                                                                 <  2:591814/139‑1 (MQ=255)                                                   tGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAAt                                                                                   >  1:414013/1‑118 (MQ=255)                                                   tGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAAt                                                                                   <  2:414013/118‑1 (MQ=255)                                                           aTGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGt                                                      >  1:334383/1‑139 (MQ=255)                                                               ccGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcgcc                                                  >  1:632910/1‑139 (MQ=255)                                                                cGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcgccg                                                 >  1:15035/1‑139 (MQ=255)                                                                                           acgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGaagaag                      <  2:632910/139‑1 (MQ=255)                                                                                            cgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgc                     <  2:486751/139‑1 (MQ=255)                                                                                                               tGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcag  <  1:520864/139‑1 (MQ=255)                                                                                                                gCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCCACGCCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcag  >  1:232082/1‑138 (MQ=255)                                                                                                                                    |                                                                                                                      CACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAG  >  NZ_CP009273/2560924‑2561172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑