BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	97,990	A→C	T247P (ACC→CCC)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	97,990	0	A	C	100.0%	137.2 / NA	37	T247P (ACC→CCC)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (21/16); total (21/16)

CCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCC  >  NZ_CP009273/97856‑98117

cctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCa                                                                                                                              <  2:60777/138‑1 (MQ=255)

   accAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGAc                                                                                                                          >  2:145347/1‑139 (MQ=255)

   accAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGAc                                                                                                                          >  1:452755/1‑139 (MQ=255)

   accAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGAc                                                                                                                          >  1:442818/1‑139 (MQ=255)

       gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTAc                                                                                                                      >  1:290647/1‑139 (MQ=255)

        ggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACg                                                                                                                     >  2:630342/1‑139 (MQ=255)

             cTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATG‑TCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCtt                                                                                                                 >  2:195677/1‑137 (MQ=255)

                           aaCAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGa                                                                                                  <  2:290647/139‑1 (MQ=255)

                            aCAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCGCGACTTACGGCTTCAGCGAAGATGCCGAc                                                                                                 <  1:190889/139‑1 (MQ=255)

                                            tttCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGAt                                                                                  >  2:225279/1‑138 (MQ=255)

                                                                 tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAgg                                                            >  1:34431/1‑139 (MQ=255)

                                                                   cGGTTGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTCCCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                           <  1:22023/138‑1 (MQ=255)

                                                                   cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                           <  1:476787/138‑1 (MQ=255)

                                                                   cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                           <  2:158578/138‑1 (MQ=255)

                                                                      tCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCAc                                                       >  2:315183/1‑139 (MQ=255)

                                                                           cGGTGATGTGTGTTGATG‑TCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTAc                                                  <  1:195677/138‑1 (MQ=255)

                                                                                aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                             >  1:157016/1‑139 (MQ=255)

                                                                                aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                             <  1:315183/139‑1 (MQ=255)

                                                                                aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctg                                              <  1:630342/138‑1 (MQ=255)

                                                                                       ttGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTaga                                                                                      <  2:374081/91‑1 (MQ=255)

                                                                                       ttGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTaga                                                                                      >  1:374081/1‑91 (MQ=255)

                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACt                                                      >  1:111219/1‑122 (MQ=255)

                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACt                                                      <  2:111219/122‑1 (MQ=255)

                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAg                                     >  1:309337/1‑139 (MQ=255)

                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAg                                     >  1:222589/1‑139 (MQ=255)

                                                                                          atgatCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGa                                   >  1:2723/1‑139 (MQ=255)

                                                                                          atgatCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGa                                   >  2:209218/1‑139 (MQ=255)

                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                             <  1:371404/124‑1 (MQ=255)

                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                                             >  2:371404/1‑124 (MQ=255)

                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcgc                                        <  2:206851/129‑1 (MQ=255)

                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcgc                                        >  1:206851/1‑129 (MQ=255)

                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                                 >  2:54886/1‑136 (MQ=255)

                                                                                                        cgcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATgc                     >  1:234492/1‑139 (MQ=255)

                                                                                                            aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt                 <  2:157016/139‑1 (MQ=255)

                                                                                                                           tGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                       <  2:419897/118‑1 (MQ=255)

                                                                                                                           tGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt                       >  1:419897/1‑118 (MQ=255)

                                                                                                                           tGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGcc  <  2:452755/139‑1 (MQ=255)

CCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCC  >  NZ_CP009273/97856‑98117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`