Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,685,950	A→T	W985R (TGG→AGG)	purL ←	phosphoribosylformylglycinamidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,685,950	0	A	T	100.0%	154.1 / NA	40	W985R (TGG→AGG)	purL	phosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/24);  total (16/24)

CATCTTCGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGCCCATGCTGTGCCAGTACGGACTC  >  NZ_CP009273/2685818‑2686085                                                                                                                                     |                                                                                                                                        caTCTTCGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGac                                                                                                                                   <  2:509729/139‑1 (MQ=255)       cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCa                                                                                                                              <  2:87575/138‑1 (MQ=255)             tatCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACgtggt                                                                                                                       <  2:459166/139‑1 (MQ=255)                    cGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCtt                                                                                                                <  2:281649/139‑1 (MQ=255)                      aCAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTc                                                                                                              <  2:296383/139‑1 (MQ=255)                                    aGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCt                                                                                                >  2:134853/1‑139 (MQ=255)                                    aGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCt                                                                                                >  2:165983/1‑139 (MQ=255)                                          ggATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGt                                                                                          <  1:539662/139‑1 (MQ=255)                                           gATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGtt                                                                                         >  2:546909/1‑139 (MQ=255)                                                gCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCgg                                                                                    >  1:43939/1‑139 (MQ=255)                                                   tcgtTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTaa                                                                                 <  2:601333/139‑1 (MQ=255)                                                          aTTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACaaa                                                                          <  1:72797/139‑1 (MQ=255)                                                              cGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCATAAAACgg                                                                      >  2:500637/1‑139 (MQ=255)                                                                                 gCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGcc                                                   <  1:590116/139‑1 (MQ=255)                                                                                  cacaCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGc                                                        >  1:213019/1‑133 (MQ=255)                                                                                  cacaCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGc                                                        <  2:213019/133‑1 (MQ=255)                                                                                   acacTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCt                                                 <  2:401746/139‑1 (MQ=255)                                                                                    cacTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa                                                >  2:190370/1‑139 (MQ=255)                                                                                            gTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATg                                         >  2:263582/1‑138 (MQ=255)                                                                                             ttGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGa                                       <  1:500637/139‑1 (MQ=255)                                                                                             ttGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGa                                       <  1:165983/139‑1 (MQ=255)                                                                                             ttGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGa                                       <  2:496328/139‑1 (MQ=255)                                                                                               gTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGc                                                                                                            >  2:1176/1‑68 (MQ=255)                                                                                               gTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGc                                                                                                            <  1:1176/68‑1 (MQ=255)                                                                                                tCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGcc                                                   <  2:218184/124‑1 (MQ=255)                                                                                                tCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGcc                                                   >  1:218184/1‑124 (MQ=255)                                                                                                 cACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGc                                                        <  2:125578/118‑1 (MQ=255)                                                                                                 cACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGc                                                        >  1:125578/1‑118 (MQ=255)                                                                                                      aGGCGCTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAg                              <  1:190370/139‑1 (MQ=255)                                                                                                        gcgcTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCa                            <  1:122482/139‑1 (MQ=255)                                                                                                          gcTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAg                          >  1:586934/1‑139 (MQ=255)                                                                                                          gcTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAg                          >  1:299950/1‑139 (MQ=255)                                                                                                          gcTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAg                          >  1:221773/1‑139 (MQ=255)                                                                                                           cTGCATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGc                         <  1:191226/139‑1 (MQ=255)                                                                                                              cATCTGCCAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGcc                        <  1:167454/137‑1 (MQ=255)                                                                                                                    ccAGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGCCCATGCtg                 <  2:43939/138‑1 (MQ=255)                                                                                                                      aGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGt                                                                                   <  1:584391/70‑1 (MQ=255)                                                                                                                      aGGTAGTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGt                                                                                   >  2:584391/1‑70 (MQ=255)                                                                                                                           gTTTCTGCCCTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGCCCATGCTGTGCCAGTa         <  2:586934/139‑1 (MQ=255)                                                                                                                                  cccTCCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGCCCATGCTGTGCCAGTACGGACTc  >  2:194184/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        CATCTTCGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATAATGGACACAATCAGCAAGCCCATGCTGTGCCAGTACGGACTC  >  NZ_CP009273/2685818‑2686085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑