Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,756,916	T→C	K533E (AAG→GAG)	abpA ←	anti‑bacteriophage protein AbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,756,916	0	T	C	100.0%	81.0 / NA	22	K533E (AAG→GAG)	abpA	anti‑bacteriophage protein AbpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/5);  total (17/5)

TCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTC  >  NZ_CP009273/2756783‑2757051                                                                                                                                      |                                                                                                                                        tcccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcat                                                                                                                                    >  2:58084/1‑139 (MQ=255) tcccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcat                                                                                                                                    >  1:59092/1‑139 (MQ=255)          aTACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAAt                                                                                                                            <  1:219363/138‑1 (MQ=255)                     ggTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGc                                                                                                                >  2:21220/1‑139 (MQ=255)                          gACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTc                                                                                                              <  2:453043/136‑1 (MQ=255)                          gACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTc                                                                                                              >  1:538039/1‑136 (MQ=255)                          gACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTc                                                                                                              >  1:453043/1‑136 (MQ=255)                             aacaGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACt                                                                                                        <  2:92706/139‑1 (MQ=255)                                            gCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAAt                                                                                         >  2:574382/1‑139 (MQ=255)                                                            aCGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCa                                                                          >  2:587993/1‑138 (MQ=255)                                                                tGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGAt                                                                     >  1:116437/1‑139 (MQ=255)                                                                         cTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACg                                                            >  1:162438/1‑139 (MQ=255)                                                                                 gCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATcc                                                    >  1:418006/1‑139 (MQ=255)                                                                                 gCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATcc                                                    >  2:599184/1‑139 (MQ=255)                                                                                               gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATaa                                                                                           >  1:22479/1‑86 (MQ=255)                                                                                               gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATaa                                                                                           <  2:22479/86‑1 (MQ=255)                                                                                                                       cGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGc              >  2:375693/1‑139 (MQ=255)                                                                                                                          ccTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGt           >  2:604071/1‑139 (MQ=255)                                                                                                                            tctctcACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGttt         >  1:232425/1‑139 (MQ=255)                                                                                                                            tctctcACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGttt         >  1:184680/1‑139 (MQ=255)                                                                                                                                tcACCTCCTCATCAACTAATTGGTTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAATATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGtttct       <  1:400359/137‑1 (MQ=255)                                                                                                                                  aCCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTttctttct   >  1:10545/1‑139 (MQ=255)                                                                                                                                   cctcctCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTtctc  <  2:418006/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTC  >  NZ_CP009273/2756783‑2757051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑