Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,786,008 T→G G459G (GGT→GGG)  gabD → NADP‑dependent succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,786,0080TG100.0% 139.6 / NA 38G459G (GGT→GGG) gabDNADP‑dependent succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/19);  total (19/19)

TGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCG  >  NZ_CP009273/2785873‑2786143
                                                                                                                                       |                                                                                                                                       
tGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                      >  1:594390/1‑139 (MQ=255)
 gccgccTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTgg                                                                                                                                     >  2:293746/1‑139 (MQ=255)
  ccgccTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTggg                                                                                                                                    >  2:169250/1‑139 (MQ=255)
                   cGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAg                                                                                                                    >  2:19268/1‑138 (MQ=255)
                            aGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCGCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATc                                                                                                           >  2:426122/1‑138 (MQ=255)
                                 cgTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGAt                                                                                                     <  2:89755/139‑1 (MQ=255)
                                 cgTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGAt                                                                                                     >  2:430441/1‑139 (MQ=255)
                                           gTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGaaa                                                                                           <  1:293746/139‑1 (MQ=255)
                                           gTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGaaa                                                                                           <  1:169250/139‑1 (MQ=255)
                                            tGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAAt                                                                                          <  2:456498/139‑1 (MQ=255)
                                              ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATc                                                                                         >  2:374583/1‑138 (MQ=255)
                                                     cgcTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAatatat                                                                                 >  2:328524/1‑139 (MQ=255)
                                                           aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:19268/139‑1 (MQ=255)
                                                           aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:430441/139‑1 (MQ=255)
                                                           aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  2:229204/139‑1 (MQ=255)
                                                           aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:609018/139‑1 (MQ=255)
                                                               cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGt                                                                       >  2:470153/1‑139 (MQ=255)
                                                               cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGt                                                                       >  2:190555/1‑139 (MQ=255)
                                                                      gtcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTaa                                                                 <  1:470153/138‑1 (MQ=255)
                                                                      gtcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTaa                                                                 <  2:460765/138‑1 (MQ=255)
                                                                             tCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGaa                                                         <  1:633114/139‑1 (MQ=255)
                                                                                 tACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGc                                                      >  1:480124/1‑138 (MQ=255)
                                                                                       cATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                               <  1:161731/139‑1 (MQ=255)
                                                                                       cATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                               <  1:190555/139‑1 (MQ=255)
                                                                                                cAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATaa                                      <  1:328524/139‑1 (MQ=255)
                                                                                                 aaTGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTc                                                                      >  2:164014/1‑106 (MQ=255)
                                                                                                 aaTGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTc                                                                      <  1:164014/106‑1 (MQ=255)
                                                                                                      aGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGtt                                                                                                                         <  1:535495/50‑1 (MQ=255)
                                                                                                      aGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGtt                                                                                                                         >  2:535495/1‑50 (MQ=255)
                                                                                                               gTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           >  2:102735/1‑87 (MQ=255)
                                                                                                               gTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           >  2:475/1‑87 (MQ=255)
                                                                                                               gTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:475/87‑1 (MQ=255)
                                                                                                               gTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:102735/87‑1 (MQ=255)
                                                                                                               gTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAgcg                       >  1:389356/1‑139 (MQ=255)
                                                                                                                ttCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAgcgc                      >  2:545666/1‑139 (MQ=255)
                                                                                                                                  tCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                      >  1:477288/1‑57 (MQ=255)
                                                                                                                                  tCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                      <  2:477288/57‑1 (MQ=255)
                                                                                                                                  tCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTccc    >  1:524600/1‑139 (MQ=255)
                                                                                                                                    ggggCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCg  <  1:426122/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCG  >  NZ_CP009273/2785873‑2786143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑