Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,831,745	A→G	I293I (ATT→ATC)	ascG ←	DNA‑binding transcriptional regulator AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,831,745	0	A	G	100.0%	150.0 / NA	40	I293I (ATT→ATC)	ascG	DNA‑binding transcriptional regulator AscG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/27);  total (13/27)

ATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCG  >  NZ_CP009273/2831615‑2831860                                                                                                                                   |                                                                                                                    aTCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTa                                                                                                              <  1:62399/138‑1 (MQ=255)  tCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACg                                                                                                            <  1:257370/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  1:607691/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  1:597742/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  2:32215/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  1:541989/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  1:442506/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  2:196166/139‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGa                                                                                             <  2:619285/138‑1 (MQ=255)                  agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGa                                                                                             <  1:510415/138‑1 (MQ=255)                   gtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAAc                                                                                           <  2:609459/139‑1 (MQ=255)                   gtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAAc                                                                                           <  2:186411/139‑1 (MQ=255)                    tgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACg                                                                                          <  1:119466/139‑1 (MQ=255)                    tgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACg                                                                                          >  2:607981/1‑139 (MQ=255)                           cggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCg                                                                                               >  1:392407/1‑127 (MQ=255)                           cggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCg                                                                                               <  2:392407/127‑1 (MQ=255)                            ggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            >  1:447281/1‑129 (MQ=255)                            ggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                            <  2:447281/129‑1 (MQ=255)                                  tCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCCTCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCaa                                                                             >  1:228404/1‑138 (MQ=255)                                  tCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAAt                                                                            <  2:552801/139‑1 (MQ=255)                                             cTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                <  1:461283/124‑1 (MQ=255)                                             cTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                >  2:461283/1‑124 (MQ=255)                                                aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGAttt                                                                                                                 <  1:257445/88‑1 (MQ=255)                                                aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGAttt                                                                                                                 >  2:257445/1‑88 (MQ=255)                                                aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGgtg                                                                                       >  2:257889/1‑114 (MQ=255)                                                aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGgtg                                                                                       <  1:257889/114‑1 (MQ=255)                                                 aGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGaa                                                             <  1:607981/139‑1 (MQ=255)                                                           cggTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACt                                                   <  2:499541/139‑1 (MQ=255)                                                              tGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAgcg                                                                                                  <  1:389725/89‑1 (MQ=255)                                                              tGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAgcg                                                                                                  >  2:389725/1‑89 (MQ=255)                                                                                 aGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTa                             >  2:86221/1‑139 (MQ=255)                                                                                   cATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGgag                                                                                                      <  1:32303/64‑1 (MQ=255)                                                                                   cATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGgag                                                                                                      >  2:32303/1‑64 (MQ=255)                                                                                             aGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc                     <  2:254882/135‑1 (MQ=255)                                                                                             aGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc                     >  1:254882/1‑135 (MQ=255)                                                                                                             ccTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCg  <  2:61411/138‑1 (MQ=255)                                                                                                                            tCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCa                                   >  2:633148/1‑90 (MQ=255)                                                                                                                            tCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCa                                   <  1:633148/90‑1 (MQ=255)                                                                                                                                cGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                             >  1:345535/1‑76 (MQ=255)                                                                                                                                cGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                             <  2:345535/76‑1 (MQ=255)                                                                                                                                   |                                                                                                                    ATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCG  >  NZ_CP009273/2831615‑2831860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑