Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,926,085	A→C	G96G (GGT→GGG)	fucO ←	lactaldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,926,085	0	A	C	100.0%	145.1 / NA	40	G96G (GGT→GGG)	fucO	lactaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/25);  total (15/25)

GGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925960‑2926219                                                                                                                              |                                                                                                                                       ggAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                            <  2:388298/138‑1 (MQ=255)            aaTCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATcc                                                                                                                >  2:409169/1‑139 (MQ=255)             aTCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                                 <  1:257176/121‑1 (MQ=255)             aTCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                                 >  2:257176/1‑121 (MQ=255)             aTCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCcg                                                                                                               <  1:353165/139‑1 (MQ=255)                       tGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                            <  2:358381/116‑1 (MQ=255)                       tGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                            >  1:358381/1‑116 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  1:172932/137‑1 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:82080/137‑1 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  1:353080/137‑1 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:331336/137‑1 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:158623/137‑1 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCt                                                                                                   >  2:98542/1‑139 (MQ=255)                         ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCt                                                                                                   >  1:68124/1‑139 (MQ=255)                              aTTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAAt                                                                                                                                <  1:602302/105‑1 (MQ=255)                              aTTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAAt                                                                                                                                >  2:602302/1‑105 (MQ=255)                                                 tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGATGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTga                                                                           >  2:604880/1‑139 (MQ=255)                                                    aGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgacga                                                                        <  1:98542/139‑1 (MQ=255)                                                       cTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAg                                                                     <  1:548995/139‑1 (MQ=255)                                                          cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  1:506065/137‑1 (MQ=255)                                                          cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  2:456051/137‑1 (MQ=255)                                                          cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  1:334690/137‑1 (MQ=255)                                                          cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTaa                                                                  >  1:182631/1‑139 (MQ=255)                                                                    cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  1:142338/139‑1 (MQ=255)                                                                    cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  2:182631/139‑1 (MQ=255)                                                                       aCTCCGGGTTGTTGATGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGttgggttggg                                                     <  1:604880/139‑1 (MQ=255)                                                                              gttgttGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              <  2:68124/139‑1 (MQ=255)                                                                                   tgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              <  2:191825/134‑1 (MQ=255)                                                                                   tgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              >  1:191825/1‑134 (MQ=255)                                                                                   tgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgtc                                         >  1:451294/1‑139 (MQ=255)                                                                                            tGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGc                                >  2:169165/1‑139 (MQ=255)                                                                                                          cAAGTATCCTGCGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTg                  <  1:426248/139‑1 (MQ=255)                                                                                                              tATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTgcagc              <  2:451294/139‑1 (MQ=255)                                                                                                              tATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTgcagc              <  2:121070/139‑1 (MQ=255)                                                                                                                tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  2:284764/1‑45 (MQ=255)                                                                                                                tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:284764/45‑1 (MQ=255)                                                                                                                tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcat            <  2:477133/139‑1 (MQ=255)                                                                                                                tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcat            >  1:477133/1‑139 (MQ=255)                                                                                                                       gagaACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCt      >  2:601502/1‑138 (MQ=255)                                                                                                                          aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATc  >  1:67428/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                       GGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925960‑2926219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑