Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,027,998	T→C	E179G (GAA→GGA)	lysS ←	lysine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,027,998	0	T	C	100.0%	69.9 / NA	20	E179G (GAA→GGA)	lysS	lysine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

ATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGC  >  NZ_CP009273/3027863‑3028115                                                                                                                                        |                                                                                                                      aTAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCAt                                                                                                                    <  2:40375/139‑1 (MQ=255)    aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCg                                                                                                                  <  2:152675/138‑1 (MQ=255)          cgcgCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTg                                                                                                               >  2:630968/1‑135 (MQ=255)                       cGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                              <  2:19697/139‑1 (MQ=255)                                       catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg                                                                               <  1:299081/138‑1 (MQ=255)                                        atcatcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgcg                                                                             <  1:496664/139‑1 (MQ=255)                                        atcatcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgcg                                                                             <  1:630968/139‑1 (MQ=255)                                            tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgcg                                                                             <  1:378067/135‑1 (MQ=255)                                            tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgcg                                                                             >  2:378067/1‑135 (MQ=255)                                            tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTc                                                                         >  1:346931/1‑139 (MQ=255)                                                 gTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGAt                                                                    >  1:408909/1‑139 (MQ=255)                                                                             ccATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg                                        >  2:221403/1‑139 (MQ=255)                                                                                       gCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                              >  1:42293/1‑75 (MQ=255)                                                                                       gCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                              <  2:42293/75‑1 (MQ=255)                                                                                                    agGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGgagatgagat                                                                                                     >  1:51384/1‑55 (MQ=255)                                                                                                    agGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGgagatgagat                                                                                                     <  2:51384/55‑1 (MQ=255)                                                                                                      gATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa               >  2:404606/1‑139 (MQ=255)                                                                                                                   aCTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc  <  2:307579/139‑1 (MQ=255)                                                                                                                               ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa               >  1:70959/1‑114 (MQ=255)                                                                                                                               ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa               <  2:70959/114‑1 (MQ=255)                                                                                                                                        |                                                                                                                      ATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGC  >  NZ_CP009273/3027863‑3028115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑