Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,034,208	T→C	R74R (CGA→CGG)	sdhE ←	FAD assembly factor SdhE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,034,208	0	T	C	100.0%	120.4 / NA	34	R74R (CGA→CGG)	sdhE	FAD assembly factor SdhE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

TGGCCAGGGCATGAGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGTCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCG  >  NZ_CP009273/3034078‑3034334                                                                                                                                   |                                                                                                                               tGGCCAGGGCATGAGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACcat                                                                                                                        >  1:498411/1‑139 (MQ=255)       gggCATGAGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTc                                                                                                                  <  1:467096/139‑1 (MQ=255)         gCATGAGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTc                                                                                                                  >  2:104107/1‑137 (MQ=255)              aGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCt                                                                                                           >  2:530604/1‑139 (MQ=255)                  aaaTAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCAt                                                                                                       >  2:438243/1‑139 (MQ=255)                        cAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTTCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCg                                                                                                 >  2:215840/1‑139 (MQ=255)                           cGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCAAAGGACCACGTTCCCGGTTCCGTGTCTGGATGCGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGtt                                                                                              >  1:72498/1‑139 (MQ=255)                                aCCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCg                                                                                         >  1:637051/1‑139 (MQ=255)                                 ccAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGt                                                                                        <  1:61267/139‑1 (MQ=255)                                           aaTCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATtcatca                                                                               <  2:337125/138‑1 (MQ=255)                                             tCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGcc                                                                            <  2:563586/139‑1 (MQ=255)                                               aGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGAcc                                                                                                                          <  2:480175/91‑1 (MQ=255)                                               aGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGAcc                                                                                                                          >  1:480175/1‑91 (MQ=255)                                               aGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAg                                                                          <  1:576523/139‑1 (MQ=255)                                                    ggAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTaaa                                                                     <  2:405851/139‑1 (MQ=255)                                                    ggAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCAGCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTaaa                                                                     <  1:215840/139‑1 (MQ=255)                                                              tgngCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGTCCACGTTCCCGGTTCCTTGTCTGTATGCGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTaaa                                                                     <  2:72498/129‑1 (MQ=255)                                                                  cgcgcCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTc                                                                                                                  <  1:584562/80‑1 (MQ=255)                                                                  cgcgcCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTc                                                                                                                  >  2:584562/1‑80 (MQ=255)                                                                          gagaCGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTc                                                >  2:147132/1‑138 (MQ=255)                                                                               gcgcAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGAAGAGCCGGa                                                                                                                            >  2:117007/1‑57 (MQ=255)                                                                               gcgcAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGAAGAGCCGGa                                                                                                                            <  1:117007/57‑1 (MQ=255)                                                                                         gATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAg                                <  2:498411/139‑1 (MQ=255)                                                                                          aTTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAg                                                                          >  2:268621/1‑96 (MQ=255)                                                                                          aTTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAg                                                                          <  1:268621/96‑1 (MQ=255)                                                                                               cacaGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGt                          >  2:548271/1‑139 (MQ=255)                                                                                               cacaGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGt                          >  1:277108/1‑139 (MQ=255)                                                                                                 caGGACCACGTTCCCGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGttt                        <  1:548271/139‑1 (MQ=255)                                                                                                       cACGTTCCCGGTTCCGTGTCTGGATGAGCCTGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTc                  <  2:25124/139‑1 (MQ=255)                                                                                                              ccGGTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCt            >  1:551483/1‑138 (MQ=255)                                                                                                                 gTTCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAg        <  1:295809/139‑1 (MQ=255)                                                                                                                   tCCGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCt      >  1:367077/1‑139 (MQ=255)                                                                                                                     cGTGTCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTg     <  1:530604/138‑1 (MQ=255)                                                                                                                       tgtCTGGATGAGCCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCg  >  2:334945/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                               TGGCCAGGGCATGAGTAAAATAACAGCGGCAACCAGCCCATGAATCAGCAAGGAAAGCCACTGTGCGCGCCAGGAGACGCGCAAATCAGATTGCCACAGGACCACGTTCCCGGTTCCGTGTCTGGATGAGTCGGACCATCATTTCCAGTTCTGCATCGGCCGGTTTACCGTGATTCATCAGCCAGTTAAACAGGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCG  >  NZ_CP009273/3034078‑3034334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑