Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,104,251	T→G	T79P (ACC→CCC)	yghD ←	GspM family type II secretion system protein YghD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,104,251	0	T	G	100.0%	133.2 / NA	36	T79P (ACC→CCC)	yghD	GspM family type II secretion system protein YghD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/14);  total (22/14)

GCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGC  >  NZ_CP009273/3104124‑3104384                                                                                                                                |                                                                                                                                      gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGcca                                                                                                                            >  2:166376/1‑139 (MQ=255)            gatgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGctgctg                                                                                                                  >  1:408403/1‑138 (MQ=255)                           cgcgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGt                                                                                                  >  1:353897/1‑139 (MQ=255)                                   cACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                          >  1:103475/1‑139 (MQ=255)                                                 aCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCCATCCGTTCGCTCAACg                                                                            <  2:177483/139‑1 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCa                                                                                                                <  2:259375/102‑1 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCa                                                                                                                >  1:259375/1‑102 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                           >  1:115251/1‑139 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                           >  2:105585/1‑139 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                           >  2:278877/1‑139 (MQ=255)                                                  cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                           >  1:372054/1‑139 (MQ=255)                                                   ccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGc                                                                          <  2:115251/139‑1 (MQ=255)                                                      cgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACggcggc                                                                       <  2:353897/139‑1 (MQ=255)                                                               cgcAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAg                                                               >  1:392085/1‑138 (MQ=255)                                                                          ttgtGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATc                                                    <  1:278877/138‑1 (MQ=255)                                                                               gCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCa                                                                                                                >  1:476090/1‑73 (MQ=255)                                                                               gCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCa                                                                                                                <  2:476090/73‑1 (MQ=255)                                                                               gCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGc                                              <  1:484127/139‑1 (MQ=255)                                                                                               cAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcaccac                              <  1:166376/139‑1 (MQ=255)                                                                                                     tatCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGc                        <  2:372054/139‑1 (MQ=255)                                                                                                       tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACg                                                                            <  1:410755/85‑1 (MQ=255)                                                                                                       tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACg                                                                            >  2:410755/1‑85 (MQ=255)                                                                                                       tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGc                        >  1:578192/1‑137 (MQ=255)                                                                                                       tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                      <  2:392085/139‑1 (MQ=255)                                                                                                         cggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACg                                               >  2:409560/1‑112 (MQ=255)                                                                                                         cggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACg                                               <  1:409560/112‑1 (MQ=255)                                                                                                         cggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAg                     >  2:225340/1‑138 (MQ=255)                                                                                                         cggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAg                     >  2:154798/1‑138 (MQ=255)                                                                                                         cggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAg                     >  1:238913/1‑138 (MQ=255)                                                                                                            cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTaa                  >  1:53535/1‑138 (MQ=255)                                                                                                            cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTaa                  >  1:464111/1‑138 (MQ=255)                                                                                                                        cAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACaccacc                             <  2:50777/115‑1 (MQ=255)                                                                                                                        cAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACaccacc                             >  1:50777/1‑115 (MQ=255)                                                                                                                           tCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgc  >  2:161487/1‑139 (MQ=255)                                                                                                                           tCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgc  <  1:584787/139‑1 (MQ=255)                                                                                                                           tCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgc  >  2:487425/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                      GCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGC  >  NZ_CP009273/3104124‑3104384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑