Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,111,922	T→C	N184D (AAT→GAT)	sslE ←	lipoprotein metalloprotease SslE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,111,922	0	T	C	100.0%	127.0 / NA	35	N184D (AAT→GAT)	sslE	lipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

AGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGA  >  NZ_CP009273/3111791‑3112057                                                                                                                                    |                                                                                                                                        agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGa                                                                                                                                  <  2:225155/139‑1 (MQ=255)  gcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGAc                                                                                                                                 <  1:205950/139‑1 (MQ=255)  gcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCGTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGGCAGACAAACCTGTTCTGTATCCGCCGGAc                                                                                                                                 <  2:215188/139‑1 (MQ=255)       gTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCt                                                                                                                             <  2:611588/138‑1 (MQ=255)             cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTg                                                                                                                      <  1:108822/139‑1 (MQ=255)                  ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGt                                                                                                                 >  2:21609/1‑139 (MQ=255)                  ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGt                                                                                                                 >  1:247268/1‑139 (MQ=255)                         gACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAg                                                                                                           <  2:594578/138‑1 (MQ=255)                          aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                       <  2:392040/109‑1 (MQ=255)                          aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                       >  1:392040/1‑109 (MQ=255)                           ccAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCg                                                                                                         >  1:387221/1‑138 (MQ=255)                             aGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                                                      <  1:607409/139‑1 (MQ=255)                             aGCTTGTTGAACTCTTCCGGGGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGGGGCGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                                                      >  2:164353/1‑139 (MQ=255)                                                                       aGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                            <  1:572579/139‑1 (MQ=255)                                                                         cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                          >  1:15704/1‑139 (MQ=255)                                                                         cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                          >  2:214138/1‑139 (MQ=255)                                                                         cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                          >  2:203760/1‑139 (MQ=255)                                                                          gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTg                                                                                                                      >  1:35447/1‑78 (MQ=255)                                                                          gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTg                                                                                                                      <  2:35447/78‑1 (MQ=255)                                                                          gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                         >  2:333922/1‑139 (MQ=255)                                                                           aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                                                        >  2:334279/1‑139 (MQ=255)                                                                               gAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                                                     <  1:21609/138‑1 (MQ=255)                                                                                   cGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGc                                                <  1:161458/139‑1 (MQ=255)                                                                                               aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                    >  1:313318/1‑139 (MQ=255)                                                                                                  aCCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                 <  1:333922/139‑1 (MQ=255)                                                                                                   ccGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                 >  1:74561/1‑138 (MQ=255)                                                                                                             cGTCAGACAAACCTGTTCTGTATCCGCCGGACAGc                                                                                                                              <  1:48396/35‑1 (MQ=255)                                                                                                             cGTCAGACAAACCTGTTCTGTATCCGCCGGACAGc                                                                                                                              >  2:48396/1‑35 (MQ=255)                                                                                                                       aCCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACTCAAGCTAcgcg            >  2:92321/1‑139 (MQ=255)                                                                                                                       aCCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg            <  2:387221/139‑1 (MQ=255)                                                                                                                            ttCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt       >  2:540736/1‑139 (MQ=255)                                                                                                                            ttCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt       >  1:472917/1‑139 (MQ=255)                                                                                                                             tCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt      >  2:436083/1‑139 (MQ=255)                                                                                                                                 tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga  <  2:472917/139‑1 (MQ=255)                                                                                                                                 tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga  >  1:611540/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        AGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGA  >  NZ_CP009273/3111791‑3112057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑