Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,114,703	A→G	intergenic (‑65/+290)	glcA ← / ← glcB	glycolate permease GlcA/malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,114,703	0	A	G	100.0%	141.9 / NA	39	intergenic (‑65/+290)	glcA/glcB	glycolate permease GlcA/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/19);  total (20/19)

GGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAAGA  >  NZ_CP009273/3114569‑3114835                                                                                                                                       |                                                                                                                                     ggATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTg                                                                                                                                   >  2:294672/1‑138 (MQ=255)  gATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGaa                                                                                                                                 <  1:235072/139‑1 (MQ=255)  gATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTACTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGaa                                                                                                                                 <  2:149550/139‑1 (MQ=255)   aTCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAAc                                                                                                                                >  1:214482/1‑139 (MQ=255)       gggCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTa                                                                                                                            <  2:297858/139‑1 (MQ=255)             ccAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAatat                                                                                                                      <  1:571908/139‑1 (MQ=255)                 agCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGc                                                                                                                  >  1:238992/1‑139 (MQ=255)                  gCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCg                                                                                                                 >  2:18178/1‑139 (MQ=255)                                                   aTTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGc                                                                                <  2:214482/139‑1 (MQ=255)                                                     ttGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCaa                                                                              >  2:50661/1‑139 (MQ=255)                                                         gTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGc                                                                          >  1:622701/1‑139 (MQ=255)                                                                     atatTGTTCAGTCGCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCAt                                                              <  1:153013/139‑1 (MQ=255)                                                                        ttGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATaa                                                            >  2:24868/1‑138 (MQ=255)                                                                        ttGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATaa                                                            >  1:295348/1‑138 (MQ=255)                                                                         tGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAAc                                                           >  1:120612/1‑138 (MQ=255)                                                                                       ttatCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTa                                             <  2:238992/138‑1 (MQ=255)                                                                                          tCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGtt                                                                                                               <  2:471731/69‑1 (MQ=255)                                                                                          tCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGtt                                                                                                               >  1:471731/1‑69 (MQ=255)                                                                                              tataTGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTc                                     >  2:86849/1‑139 (MQ=255)                                                                                              tataTGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTc                                     >  1:549995/1‑139 (MQ=255)                                                                                                           taCTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacg                        <  2:622701/139‑1 (MQ=255)                                                                                                            aCTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTa                                             <  1:79020/117‑1 (MQ=255)                                                                                                            aCTTAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTa                                             >  2:79020/1‑117 (MQ=255)                                                                                                               tAAGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcaga                    >  1:6670/1‑139 (MQ=255)                                                                                                                 aGGTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacg                  <  1:18178/139‑1 (MQ=255)                                                                                                                   gTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacgca                <  2:554820/139‑1 (MQ=255)                                                                                                                   gTTGTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacg                  >  2:262032/1‑137 (MQ=255)                                                                                                                      gTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacg                  >  1:177164/1‑134 (MQ=255)                                                                                                                      gTAATAAGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacg                  <  2:177164/134‑1 (MQ=255)                                                                                                                       taataaGCCAAAGAGGGCTGAACTGTAAAATATAGGCGTta                                                                                                              <  1:552651/41‑1 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTta                                                                                                              >  2:552651/1‑41 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCaa                                                                                                 <  1:187972/54‑1 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCaa                                                                                                 >  2:187972/1‑54 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACa                                                          <  1:395859/93‑1 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACa                                                          >  2:395859/1‑93 (MQ=255)                                                                                                                       taataaGCAAAAGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCagag             <  2:549995/138‑1 (MQ=255)                                                                                                                                aaaGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAAg   <  1:50661/139‑1 (MQ=255)                                                                                                                                 aaGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAAGTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGtt      >  1:391335/1‑135 (MQ=255)                                                                                                                                 aaGAGGGCTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAAGTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAAGa  <  2:391335/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAAGA  >  NZ_CP009273/3114569‑3114835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑