Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,246,701	A→G	I291I (ATT→ATC)	yhaJ ←	DNA‑binding transcriptional regulator YhaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,246,701	0	A	G	100.0%	69.8 / NA	20	I291I (ATT→ATC)	yhaJ	DNA‑binding transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

AAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCA  >  NZ_CP009273/3246571‑3246831                                                                                                                                   |                                                                                                                                   aaGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCg                                                                                                                            >  1:396678/1‑139 (MQ=255)                   aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                          <  1:198020/139‑1 (MQ=255)                   aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                          <  1:634494/139‑1 (MQ=255)                         aCGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTccc                                                                                                     <  1:311119/138‑1 (MQ=255)                                              gTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                               <  2:345859/139‑1 (MQ=255)                                                             aGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAatat                                                                    <  2:256686/135‑1 (MQ=255)                                                                    gggCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTg                                                         >  1:90101/1‑139 (MQ=255)                                                                         atatTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGa                                                    >  2:628823/1‑139 (MQ=255)                                                                                          attaACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGaca                                    <  1:628823/138‑1 (MQ=255)                                                                                               cAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAAc                              <  2:396678/139‑1 (MQ=255)                                                                                                          attattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                          >  1:384274/1‑52 (MQ=255)                                                                                                          attattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                          <  2:384274/52‑1 (MQ=255)                                                                                                          attattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCa                                                                                          <  1:557894/68‑1 (MQ=255)                                                                                                          attattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCa                                                                                          >  2:557894/1‑68 (MQ=255)                                                                                                            tattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACAcc                                                                                                                    >  2:198295/1‑40 (MQ=255)                                                                                                            tattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACAcc                                                                                                                    <  1:198295/40‑1 (MQ=255)                                                                                                            tattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCaa                 >  2:494891/1‑139 (MQ=255)                                                                                                                   cGTTAAAAAGGTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGAAACCGCCCTTCCGCAATATCttt          >  1:638349/1‑139 (MQ=255)                                                                                                                      tAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTc       >  1:192130/1‑139 (MQ=255)                                                                                                                           aGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCTTTCCGCAATATCTTTTTCGACCa  <  2:90101/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   AAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCA  >  NZ_CP009273/3246571‑3246831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑