Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	175,042	T→C	D200G (GAT→GGT)	mtnN ←	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	175,042	0	T	C	100.0%	111.8 / NA	31	D200G (GAT→GGT)	mtnN	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

AAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAA  >  NZ_CP009273/174909‑175174                                                                                                                                      |                                                                                                                                     aaaGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGcc                                                                                                                                 <  2:348496/139‑1 (MQ=255)         ggCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCg                                                                                                                           >  2:383636/1‑137 (MQ=255)         ggCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCg                                                                                                                           >  1:364960/1‑137 (MQ=255)                                    aGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGa                                                                                               <  2:364960/138‑1 (MQ=255)                                          gtgCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGa                                                                                         >  2:490265/1‑138 (MQ=255)                                            gCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGgaga                                                                                                                       <  2:534811/106‑1 (MQ=255)                                            gCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGgaga                                                                                                                       >  1:534811/1‑106 (MQ=255)                                                   tCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTgg                                                                                 >  1:516644/1‑137 (MQ=255)                                                    cTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCa                                                                               <  2:62344/138‑1 (MQ=255)                                                               aCTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGTACGTTGAAATTGTGGCa                                                                               <  1:140940/127‑1 (MQ=255)                                                               aCTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCa                                                                               >  2:140940/1‑127 (MQ=255)                                                                       aTCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCg                                                           <  1:490265/139‑1 (MQ=255)                                                                             cTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGcc                                                                                                                                 <  2:529599/63‑1 (MQ=255)                                                                             cTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGcc                                                                                                                                 >  1:529599/1‑63 (MQ=255)                                                                                     tttAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCg                                                              <  1:507791/122‑1 (MQ=255)                                                                                     tttAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCg                                                              >  2:507791/1‑122 (MQ=255)                                                                                      ttAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAg                                            >  1:587058/1‑139 (MQ=255)                                                                                       tAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAg                                            >  2:332735/1‑138 (MQ=255)                                                                                         gCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCaa                                         <  1:133767/139‑1 (MQ=255)                                                                                          cGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAAt                                        <  2:211864/139‑1 (MQ=255)                                                                                             cAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGc                                     <  2:57246/139‑1 (MQ=255)                                                                                               aCAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCAt                                                    <  2:288057/122‑1 (MQ=255)                                                                                               aCAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCAt                                                    >  1:288057/1‑122 (MQ=255)                                                                                               aCAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCt                                   >  1:248680/1‑139 (MQ=255)                                                                                                             aTCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTgg                                                                                 <  1:563510/79‑1 (MQ=255)                                                                                                             aTCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTgg                                                                                 >  2:563510/1‑79 (MQ=255)                                                                                                             aTCGAAGCTAAGATGAGACTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTgg                     >  1:379422/1‑139 (MQ=255)                                                                                                                              aCTGTTGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGAc     >  2:321276/1‑138 (MQ=255)                                                                                                                                  ttGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAAt                                        <  2:271399/99‑1 (MQ=255)                                                                                                                                  ttGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAAt                                        >  1:271399/1‑99 (MQ=255)                                                                                                                                  ttGACCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCaa  >  1:621726/1‑137 (MQ=255)                                                                                                                                      |                                                                                                                                     AAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAA  >  NZ_CP009273/174909‑175174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑