| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,332,009 | A→G | V200A (GTC→GCC) | mlaE ← | lipid asymmetry maintenance ABC transporter permease subunit MlaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,332,009 | 0 | A | G | 100.0% | 84.0 / NA | 24 | V200A (GTC→GCC) | mlaE | lipid asymmetry maintenance ABC transporter permease subunit MlaE |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/15); total (9/15) | |||||||||||
GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTGGT > NZ_CP009273/3331874‑3332143 | gCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCa < 2:625134/139‑1 (MQ=255) ccAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAg < 1:426392/139‑1 (MQ=255) cGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATa < 1:22146/139‑1 (MQ=255) gTGGACAACGGGGCGAGTGGTTGCCCGGCTAATCACGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGGGATGGCGAACACCACGCTCTTAATCAGACAGGTGGCCAGATCCATACGc > 2:386729/1‑139 (MQ=255) cGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGAc > 1:163570/1‑139 (MQ=255) ggTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACg < 2:549179/139‑1 (MQ=255) tGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACg < 2:459612/127‑1 (MQ=255) tGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACg > 1:459612/1‑127 (MQ=255) cGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCa < 2:636170/138‑1 (MQ=255) cGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAAACCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCa < 2:228961/138‑1 (MQ=255) ttGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCAt > 2:412683/1‑139 (MQ=255) tGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGCACACCACGCTCCTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCAtt > 1:261072/1‑139 (MQ=255) cccGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTg < 1:409435/138‑1 (MQ=255) aGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCGCGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATGTTGCATTGCCGACCagaagacaccg < 2:151394/139‑6 (MQ=255) gTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTAt < 1:531505/139‑1 (MQ=255) tAAACAACGAAATCTACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg > 2:261293/1‑113 (MQ=255) tAAACAACGAAATCTACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg < 1:261293/113‑1 (MQ=255) aaaCAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGcc < 1:412683/138‑1 (MQ=255) aacGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa < 2:441505/139‑1 (MQ=255) caccacGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGcgac > 2:112247/1‑138 (MQ=255) gCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGaaaa < 2:163570/137‑1 (MQ=255) aCAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAAt > 2:383332/1‑69 (MQ=255) aCAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAAt < 1:383332/69‑1 (MQ=255) ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTGGt > 1:538542/1‑138 (MQ=255) | GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTGGT > NZ_CP009273/3331874‑3332143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |