Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,473,413	A→G	M185T (ATG→ACG)	kefB ←	glutathione‑regulated potassium‑efflux system protein KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,473,413	0	A	G	100.0%	113.3 / NA	31	M185T (ATG→ACG)	kefB	glutathione‑regulated potassium‑efflux system protein KefB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/12);  total (19/12)

ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATT  >  NZ_CP009273/3473279‑3473546                                                                                                                                       |                                                                                                                                      aTGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                   <  2:377204/139‑1 (MQ=255)  tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                   >  1:114686/1‑138 (MQ=255)  tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                   >  1:441938/1‑138 (MQ=255)                         gcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                   <  2:403780/115‑1 (MQ=255)                         gcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                   >  1:403780/1‑115 (MQ=255)                            tCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTc                                                                                                        >  1:315943/1‑139 (MQ=255)                                              cccGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCcaacaa                                                                                      >  2:557628/1‑139 (MQ=255)                                                cGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCTACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACg                                                                                    <  1:334543/139‑1 (MQ=255)                                                     gCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt                                                                               <  2:34597/139‑1 (MQ=255)                                                        aGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCACCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAAc                                                                            >  1:463309/1‑139 (MQ=255)                                                          aaGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCTTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                          <  1:557628/139‑1 (MQ=255)                                                                       gCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACATTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGAt                                                              >  1:7709/1‑138 (MQ=255)                                                                          gAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACt                                                          >  2:344851/1‑139 (MQ=255)                                                                                cGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                          <  2:389551/117‑1 (MQ=255)                                                                                cGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                          >  1:389551/1‑117 (MQ=255)                                                                                  gACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATc                                                  >  1:20540/1‑139 (MQ=255)                                                                                     gCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc                                                                                                                         >  1:122708/1‑65 (MQ=255)                                                                                     gCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc                                                                                                                         <  2:122708/65‑1 (MQ=255)                                                                                       aGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGa                                              >  1:127576/1‑138 (MQ=255)                                                                                         tAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGaaa                                            >  2:426952/1‑138 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAAc                                   >  1:335224/1‑139 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCT‑‑GATTACTGCCAGATCCTGAAACAGCAGAAcc                                  >  2:170836/1‑138 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCT‑‑GATTACTGCCAGATCCTGAAACAGCAGAACCg                                 <  1:170836/139‑1 (MQ=255)                                                                                                              cATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCa                                                                                                                           <  2:339893/38‑1 (MQ=255)                                                                                                              cATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCa                                                                                                                           >  1:339893/1‑38 (MQ=255)                                                                                                              cATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGc                      >  2:191311/1‑139 (MQ=255)                                                                                                                 gCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg                       <  1:501059/135‑1 (MQ=255)                                                                                                                 gCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg                       >  2:501059/1‑135 (MQ=255)                                                                                                                            ccAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCt        >  2:98791/1‑139 (MQ=255)                                                                                                                                 aCCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGAt   <  2:214833/139‑1 (MQ=255)                                                                                                                                  ccTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGAtt  <  1:293615/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATT  >  NZ_CP009273/3473279‑3473546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑