Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,572,235	A→G	intergenic (‑149/+75)	gntR ← / ← yhhW	gluconate operon transcriptional repressor GntR/quercetin 2,3‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,572,235	0	A	G	100.0%	129.6 / NA	36	intergenic (‑149/+75)	gntR/yhhW	gluconate operon transcriptional repressor GntR/quercetin 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/15);  total (21/15)

ACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGAAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCATGGA  >  NZ_CP009273/3572099‑3572362                                                                                                                                         |                                                                                                                                aCAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAg                                                                                                                               >  1:238162/1‑139 (MQ=255) aCAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAg                                                                                                                               >  2:621812/1‑139 (MQ=255)             gatTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCa                                                                                                                   <  1:456537/139‑1 (MQ=255)                  tctTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGa                                                                                                              >  2:147717/1‑139 (MQ=255)                            acacGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGgagcgag                                                                                                    >  1:81384/1‑139 (MQ=255)                            acacGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGgagcgag                                                                                                    >  1:529912/1‑139 (MQ=255)                                 gACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGcgagcgag                                                                                                >  1:597035/1‑138 (MQ=255)                                                tCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAagg                                                                                >  2:235360/1‑139 (MQ=255)                                                  ccGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAaggca                                                                              <  2:597035/139‑1 (MQ=255)                                                  ccGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAaggc                                                                               <  2:600440/138‑1 (MQ=255)                                                              gTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGAt                                                                  >  1:553404/1‑139 (MQ=255)                                                               tGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATg                                                                 >  1:564629/1‑139 (MQ=255)                                                                 cTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGcc                                                               >  2:122922/1‑139 (MQ=255)                                                                     tttACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGtt                                                            <  1:122922/138‑1 (MQ=255)                                                                               ttaGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAAc                                                 <  2:81384/139‑1 (MQ=255)                                                                                      taCACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACcggcgg                                           >  2:69368/1‑138 (MQ=255)                                                                                      taCACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACcggcgg                                           >  2:515808/1‑138 (MQ=255)                                                                                       acacTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACcggcgg                                           >  2:556708/1‑137 (MQ=255)                                                                                          cTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGAt                                      <  2:466905/139‑1 (MQ=255)                                                                                            aaaTCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATc                                     >  1:528328/1‑138 (MQ=255)                                                                                             aaTCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCg                                    >  1:531800/1‑138 (MQ=255)                                                                                              aTCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGaa                                  <  1:235360/139‑1 (MQ=255)                                                                                              aTCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGaa                                  <  2:193894/139‑1 (MQ=255)                                                                                              aTCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGaa                                  >  1:582571/1‑139 (MQ=255)                                                                                                   ttGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGt                              >  2:98710/1‑138 (MQ=255)                                                                                                           cATTGTGCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCg                     >  2:416747/1‑139 (MQ=255)                                                                                                                 gCTAACGACGCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCg               >  2:269699/1‑139 (MQ=255)                                                                                                                     acgacgCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGcgagcgag                                                                                                >  2:324074/1‑54 (MQ=255)                                                                                                                     acgacgCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGcgagcgag                                                                                                <  1:324074/54‑1 (MQ=255)                                                                                                                     acgacgCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCt             <  2:201925/137‑1 (MQ=255)                                                                                                                        acgCGGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCg        <  1:606095/139‑1 (MQ=255)                                                                                                                          gcgGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCa      >  2:307850/1‑139 (MQ=255)                                                                                                                          gcgGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCa      <  1:98710/139‑1 (MQ=255)                                                                                                                          gcgGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCa      <  1:556708/139‑1 (MQ=255)                                                                                                                          gcgGCAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCa      <  1:439376/139‑1 (MQ=255)                                                                                                                              cAAAACCCCGGGAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCatgga  <  2:564629/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                ACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAAAACAGTGCTTGTTTACAATTATTAGATATACACTAAATCAATTGCATCGCATTGTGCTAACGACGCGGCAAAACCCCGGAAGCGTACATCAGCACGCGACCGGAGCGAGCGAGGAAGCCTGCAAAAAGGCAGGCTTCAAAGATGCCGTTGAGTTTTAAACCGGCGGCAGATCGAACAGTAACACTTCGCTGTCGCTATCCGCATGGA  >  NZ_CP009273/3572099‑3572362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑