Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,639,274	T→C	V177A (GTC→GCC)	rlmJ →	23S rRNA (adenine(2030)‑N(6))‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,639,274	0	T	C	100.0%	117.0 / NA	32	V177A (GTC→GCC)	rlmJ	23S rRNA (adenine(2030)‑N(6))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

GCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCA  >  NZ_CP009273/3639148‑3639396                                                                                                                                |                                                                                                                           gCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAg                                                                                                                 >  1:608418/1‑138 (MQ=255)   cGTGCGCGGGTCAAAAAAGCCGCGGTTTTCCAGCAGCTAAAGCCCAAC‑CTCCCGCCGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAg                                                                                                              <  2:412204/139‑1 (MQ=255)    gTGCGGGTGTCAAAAACCCCGACGGTTTCCAGCATCTAAGGCCCAAC‑CTGCCCCCGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGa                                                                                                             <  2:409279/139‑1 (MQ=255)     tGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAg                                                                                                              >  1:387411/1‑137 (MQ=255)            gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTAc                                                                                                      >  2:138707/1‑138 (MQ=255)                        gACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCa                                                                                         >  2:102030/1‑139 (MQ=255)                               ccaagcagcTAAAGCCAAAC‑CGGCGGCCGTTTCCCCGCCGTGGTTTAACCCTTACCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                                   <  2:139247/135‑1 (MQ=255)                                 cagcagCTTAAGGCAAAC‑CGGCCGCGGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                                <  1:13396/139‑1 (MQ=255)                                    cagcTTAAGGCCAAA‑CTGCCGCCGGTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                             <  1:73971/139‑1 (MQ=255)                                      gcTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                           <  1:102030/139‑1 (MQ=255)                                       cTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCAc                                                                          >  1:561900/1‑139 (MQ=255)                                       cTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCAc                                                                          >  1:139247/1‑139 (MQ=255)                                       cTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCAc                                                                          >  1:248313/1‑139 (MQ=255)                                           agccaaaa‑CTGCCGCGGTTTCCCCCCCGTGGTTTAATCCTTACCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                                      <  2:174493/134‑1 (MQ=255)                                            ggCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAGCTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTgg                                                                     >  2:169714/1‑139 (MQ=255)                                            agCCAAACCTGCCGCCGTTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                                      <  1:568781/138‑1 (MQ=255)                                                 aa‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcc                                                                >  2:313172/1‑139 (MQ=255)                                                                  ccgccgTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAg                                                <  2:387411/139‑1 (MQ=255)                                                                        tGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                           >  2:315205/1‑138 (MQ=255)                                                                        tGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                           >  1:59967/1‑138 (MQ=255)                                                                          gTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                                          >  2:634467/1‑137 (MQ=255)                                                                               aTCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                                   <  1:313172/139‑1 (MQ=255)                                                                                    tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                              >  1:60987/1‑139 (MQ=255)                                                                                     aTCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                              >  1:613713/1‑138 (MQ=255)                                                                                      tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                            <  1:315205/139‑1 (MQ=255)                                                                                      tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                            <  2:248313/139‑1 (MQ=255)                                                                                      tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                            <  1:215814/139‑1 (MQ=255)                                                                                                                 aCTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTc                                                  >  2:624167/1‑90 (MQ=255)                                                                                                                 aCTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTc                                                  <  1:624167/90‑1 (MQ=255)                                                                                                                 aCTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCa  >  2:586538/1‑138 (MQ=255)                                                                                                                         gCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                            <  1:227448/104‑1 (MQ=255)                                                                                                                         gCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                            >  2:227448/1‑104 (MQ=255)                                                                                                                             tggcCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                                  >  1:594421/1‑94 (MQ=255)                                                                                                                             tggcCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                                  <  2:594421/94‑1 (MQ=255)                                                                                                                                |                                                                                                                           GCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCA  >  NZ_CP009273/3639148‑3639396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑