Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,714,265	A→G	I76V (ATC→GTC)	BW25113_RS25300 →	IS3‑like element IS150 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,714,265	0	A	G	100.0%	195.3 / NA	52	I76V (ATC→GTC)	BW25113_RS25300	IS3‑like element IS150 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/27);  total (25/27)

TTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTCAAGATTGGCA  >  NZ_CP009273/3714132‑3714394                                                                                                                                      |                                                                                                                                  ttttGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGag                                                                                                                              >  2:631506/1‑139 (MQ=255)           ccccGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGt                                                                                                                    >  2:584592/1‑139 (MQ=255)             ccGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTcc                                                                                                                  <  2:182078/139‑1 (MQ=255)                     aGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAAt                                                                                                            >  2:564995/1‑137 (MQ=255)                     aGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCa                                                                                                          <  2:4375/139‑1 (MQ=255)                      ggTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAg                                                                                                         >  2:354239/1‑139 (MQ=255)                            gACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACAt                                                                                                                      <  1:154315/120‑1 (MQ=255)                            gACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACAt                                                                                                                      >  2:154315/1‑120 (MQ=255)                              cATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgc                                                                                                 <  2:435720/139‑1 (MQ=255)                              cATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgc                                                                                                 <  1:564995/139‑1 (MQ=255)                               aTGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                <  1:331192/139‑1 (MQ=255)                               aTGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                <  1:631506/139‑1 (MQ=255)                               aTGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                <  1:622049/139‑1 (MQ=255)                               aTGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                <  1:305835/139‑1 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGagc                                                                                                                             >  2:408643/1‑103 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGagc                                                                                                                             <  1:408643/103‑1 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                >  2:560097/1‑132 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctgctgct                                                                                                <  1:560097/132‑1 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTa                                                                                         >  2:568545/1‑139 (MQ=255)                                      gCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTa                                                                                         >  2:583436/1‑139 (MQ=255)                                        ccTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtg                                                                                       <  2:71905/139‑1 (MQ=255)                                        ccTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtg                                                                                       <  1:397383/139‑1 (MQ=255)                                        ccTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtg                                                                                       <  1:364698/139‑1 (MQ=255)                                            tATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtgcttg                                                                                   >  2:461124/1‑139 (MQ=255)                                              tGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtgcttgct                                                                                 <  1:176724/139‑1 (MQ=255)                                                 aaaaCATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTgg                                                                               >  1:525289/1‑138 (MQ=255)                                                        ggAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGGTAGTGCTGATCCAGAGTTGCGTATTAATGTCGTGGAAGGTGTGGTCGGGCAGCACATGTCCCTTAAGCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGtt                                                                       >  1:140866/1‑139 (MQ=255)                                                         gagaAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGtt                                                                       <  2:200236/138‑1 (MQ=255)                                                          agaAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCt                                                                     <  1:338517/139‑1 (MQ=255)                                                                  gTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAg                                                             <  2:223255/139‑1 (MQ=255)                                                                                          ttAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAgcg                                     >  1:205268/1‑139 (MQ=255)                                                                                          ttAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAgcg                                     <  2:213021/139‑1 (MQ=255)                                                                                            aGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGcgcg                                   >  1:500449/1‑139 (MQ=255)                                                                                                 tGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGgag                                >  2:125416/1‑137 (MQ=255)                                                                                                  gATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAg                             >  1:577719/1‑139 (MQ=255)                                                                                                       agagTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtgcttgct                                                                                 >  2:280451/1‑82 (MQ=255)                                                                                                       agagTTGCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTAtgcttgct                                                                                 <  1:280451/82‑1 (MQ=255)                                                                                                             gCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctg                                                                                                     >  2:34351/1‑56 (MQ=255)                                                                                                             gCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGgctg                                                                                                     <  1:34351/56‑1 (MQ=255)                                                                                                             gCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTAcgc                  >  1:256905/1‑139 (MQ=255)                                                                                                             gCGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTAcg                   <  2:500449/138‑1 (MQ=255)                                                                                                             gCGTATTAAGGTCGTGAAAGCTGTGGTAGAGAAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGAAGAAGCTGGTAAAcgc                  >  2:95195/1‑139 (MQ=255)                                                                                                              cGTATTAAGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTAcgcg                 >  2:599661/1‑139 (MQ=255)                                                                                                              cGTATTAAGGTCGTAAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGaa                                                    >  2:29466/1‑104 (MQ=255)                                                                                                              cGTATTAAGGTCGTAAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGaa                                                    <  1:29466/104‑1 (MQ=255)                                                                                                                    aaGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTc            <  2:577719/138‑1 (MQ=255)                                                                                                                     aGGTCGTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTc            >  1:532931/1‑137 (MQ=255)                                                                                                                          gTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTAcgcg                 >  2:585781/1‑127 (MQ=255)                                                                                                                          gTGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTAcgcg                 <  1:585781/127‑1 (MQ=255)                                                                                                                           tGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTCAAGATTgg    <  2:532931/139‑1 (MQ=255)                                                                                                                           tGAAAGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGATTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTCAAGATTgg    >  2:254539/1‑139 (MQ=255)                                                                                                                             aaaGCTGTGGTCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTCAAGATTGGCa  <  1:262799/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCGCGGAGAAGCTGGTTTACGCGCGCTCAAGATTGGCA  >  NZ_CP009273/3714132‑3714394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑