| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,747,593 | C→G | A66P (GCC→CCC) | yiaW ← | DUF3302 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,747,593 | 0 | C | G | 98.5% | 246.6 / ‑3.6 | 68 | A66P (GCC→CCC) | yiaW | DUF3302 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (28/39); total (28/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGAT > NZ_CP009273/3747465‑3747725 | ttACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCca > 1:59264/1‑138 (MQ=255) cTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACaaa < 2:22750/139‑1 (MQ=255) ggCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAAc > 2:347088/1‑139 (MQ=255) cAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg > 2:517483/1‑139 (MQ=255) agaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGc < 1:589635/139‑1 (MQ=255) agaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGc < 2:59264/139‑1 (MQ=255) gaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc < 2:278080/139‑1 (MQ=255) gaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc < 2:581664/139‑1 (MQ=255) gagGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGa < 1:379908/139‑1 (MQ=255) gATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATaa > 1:262162/1‑139 (MQ=255) gATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATg > 2:149160/1‑138 (MQ=255) aTGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt < 2:275668/138‑1 (MQ=255) aTGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt < 1:625328/138‑1 (MQ=255) tGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTaa < 1:38276/139‑1 (MQ=255) tGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTaa < 2:87663/139‑1 (MQ=255) cGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt > 1:19242/1‑134 (MQ=255) cGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt < 2:19242/134‑1 (MQ=255) cGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACg > 2:453276/1‑138 (MQ=255) ggCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGt > 2:78657/1‑138 (MQ=255) cTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGa < 1:78657/139‑1 (MQ=255) cTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGa < 1:327482/139‑1 (MQ=255) tttCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTc < 2:377976/139‑1 (MQ=255) tctcCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCa < 1:201176/138‑1 (MQ=255) ctcCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCAc < 1:149160/138‑1 (MQ=255) gCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc > 2:209412/1‑138 (MQ=255) gCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc > 2:511906/1‑138 (MQ=255) gCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc > 2:280341/1‑138 (MQ=255) gCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc > 1:601707/1‑138 (MQ=255) cGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGc < 1:354839/139‑1 (MQ=255) cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc < 2:590796/127‑1 (MQ=255) cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAAc > 1:590796/1‑127 (MQ=255) cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGaa < 1:313545/139‑1 (MQ=255) ttgcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTaaa < 2:627269/108‑1 (MQ=255) ttgcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTaaa > 1:627269/1‑108 (MQ=255) ttgcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCg > 1:137931/1‑139 (MQ=255) gcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt < 2:358325/138‑1 (MQ=255) gcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCGCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt < 2:157340/138‑1 (MQ=255) gttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg > 2:103286/1‑82 (MQ=255) gttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg < 1:103286/82‑1 (MQ=255) gttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCgg > 2:573927/1‑138 (MQ=255) gcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCAt > 2:358921/1‑138 (MQ=255) aTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAg < 2:266815/139‑1 (MQ=255) gATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTaa < 1:479211/86‑1 (MQ=255) gATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTaa > 2:479211/1‑86 (MQ=255) gCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt > 1:209539/1‑113 (MQ=255) gCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt < 2:209539/113‑1 (MQ=255) gCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTg > 2:606740/1‑139 (MQ=255) tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCaa > 1:286152/1‑139 (MQ=255) tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCa < 1:606740/138‑1 (MQ=255) cATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATc < 1:573927/139‑1 (MQ=255) ttCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAg < 1:157867/139‑1 (MQ=255) ccccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAgg > 1:22613/1‑138 (MQ=255) cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGt < 1:511906/138‑1 (MQ=255) cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGt < 1:280341/138‑1 (MQ=255) cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGt < 1:209412/138‑1 (MQ=255) cATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAgg < 2:621493/139‑1 (MQ=255) cGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAatat > 1:5853/1‑139 (MQ=255) cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAgg < 1:51851/127‑1 (MQ=255) cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAgg > 2:51851/1‑127 (MQ=255) cGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATg < 2:262162/138‑1 (MQ=255) ggCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGga > 1:464981/1‑98 (MQ=255) ggCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGga < 2:464981/98‑1 (MQ=255) ggCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCa < 2:496770/116‑1 (MQ=255) ggCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCa > 1:496770/1‑116 (MQ=255) aaaGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAaataat > 2:432549/1‑139 (MQ=255) aaGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAaataat < 1:358921/138‑1 (MQ=255) aGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGa < 1:453276/139‑1 (MQ=255) gCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGAt < 1:432549/139‑1 (MQ=255) | TTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGAT > NZ_CP009273/3747465‑3747725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |