Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,969,741 C→T A35V (GCG→GTG)  wzxE → lipid III flippase WzxE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,969,7410CT96.4% 98.0 / ‑6.6 28A35V (GCG→GTG) wzxElipid III flippase WzxE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/19);  minor base G (1/0);  total (9/19)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

ACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTG  >  NZ_CP009273/3969613‑3969874
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aCTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCTTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTggg                                                                                                                             <  1:252600/139‑1 (MQ=255)
  tttGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCt                                                                                                                           >  2:589961/1‑139 (MQ=255)
    tgttgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTgg                                                                                                                         >  1:24657/1‑139 (MQ=255)
              ctTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGACAAAttt                                                                                                               >  1:412628/1‑139 (MQ=255)
                              gTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTAc                                                                                                <  2:54033/138‑1 (MQ=255)
                                                        cggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                                     >  2:513148/1‑139 (MQ=255)
                                                         ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                                                    <  1:329917/139‑1 (MQ=255)
                                                         ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                                                    <  2:572314/139‑1 (MQ=255)
                                                         ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                                                    <  2:24657/139‑1 (MQ=255)
                                                         ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                                                    <  2:188946/139‑1 (MQ=255)
                                                                 cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTa                                                             <  1:43565/138‑1 (MQ=255)
                                                                    tGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                                                         >  2:553484/1‑139 (MQ=255)
                                                                     ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGGTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                                                        >  1:219201/1‑139 (MQ=255)
                                                                      gTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                            <  1:363918/134‑1 (MQ=255)
                                                                      gTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                            >  2:363918/1‑134 (MQ=255)
                                                                       tCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtgt                                                      <  1:504898/139‑1 (MQ=255)
                                                                                  gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACg                                           <  2:278248/139‑1 (MQ=255)
                                                                                   ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACg                                           <  1:440998/139‑1 (MQ=255)
                                                                                     gggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                            <  1:495711/119‑1 (MQ=255)
                                                                                     gggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGGGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                            >  2:495711/1‑119 (MQ=255)
                                                                                       gTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                      <  2:374454/139‑1 (MQ=255)
                                                                                       gTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                      <  1:527337/139‑1 (MQ=255)
                                                                                       gTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGACAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                      <  2:412628/139‑1 (MQ=255)
                                                                                        ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc                                     <  1:589961/139‑1 (MQ=255)
                                                                                        ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc                                     <  1:553484/139‑1 (MQ=255)
                                                                                        ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc                                     <  1:532418/139‑1 (MQ=255)
                                                                                           cTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAg                                   <  1:78157/138‑1 (MQ=255)
                                                                                                                           t‑CCGGTGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTg  >  1:606222/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                     
ACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGT‑CCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTG  >  NZ_CP009273/3969613‑3969874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑