Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,091,145	C→T	G136G (GGC→GGT)	sodA →	superoxide dismutase [Mn]

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,091,145	0	C	T	100.0%	108.6 / NA	30	G136G (GGC→GGT)	sodA	superoxide dismutase [Mn]
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

CCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACC  >  NZ_CP009273/4091019‑4091278                                                                                                                               |                                                                                                                                      ccTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCg                                                                                                                           <  1:265646/139‑1 (MQ=255) ccTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCg                                                                                                                           <  2:592189/139‑1 (MQ=255)         gTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTc                                                                                                                   <  1:179316/139‑1 (MQ=255)           gACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGGTTCTa                                                                                                                 >  2:35892/1‑139 (MQ=255)                  aaGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTaa                                                                                                          >  2:611234/1‑139 (MQ=255)                        cTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAAACAGGa                                                                                                    >  1:600206/1‑139 (MQ=255)                             gAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATtctc                                                                                               <  1:85634/139‑1 (MQ=255)                             gAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATtct                                                                                                <  2:344325/138‑1 (MQ=255)                                  tGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCt                                                                                           >  1:361013/1‑138 (MQ=255)                                     cTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTTTCCGCTGATg                                                                                       >  1:497808/1‑139 (MQ=255)                                                          cAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCt                                                                  >  1:235774/1‑139 (MQ=255)                                                          cAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCt                                                                  >  1:52141/1‑139 (MQ=255)                                                                  aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTTTGGCGCTTCCGGCtt                                                          >  2:579059/1‑139 (MQ=255)                                                                  aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCtt                                                          >  2:332671/1‑139 (MQ=255)                                                                          aaaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTa                                                  <  2:79617/139‑1 (MQ=255)                                                                          aaaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTa                                                  <  2:23561/139‑1 (MQ=255)                                                                           aaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTAt                                                 <  1:164985/139‑1 (MQ=255)                                                                              cGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATggg                                              >  2:629047/1‑139 (MQ=255)                                                                                 cAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCt                                           <  2:52141/139‑1 (MQ=255)                                                                                  aGCTGCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTg                                          <  2:189558/139‑1 (MQ=255)                                                                                              tGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTggg                                <  1:629047/137‑1 (MQ=255)                                                                                                gTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAAc                             <  1:579059/138‑1 (MQ=255)                                                                                                gTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAAc                             <  1:287679/138‑1 (MQ=255)                                                                                                    cGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCt                        <  2:497808/139‑1 (MQ=255)                                                                                                      gCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGt                                                                                    >  1:551151/1‑77 (MQ=255)                                                                                                      gCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGt                                                                                    <  2:551151/77‑1 (MQ=255)                                                                                                      gCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTta                      <  1:611234/139‑1 (MQ=255)                                                                                                          ggCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTtactac                  >  1:340667/1‑139 (MQ=255)                                                                                                                     tGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTc         >  1:164061/1‑137 (MQ=255)                                                                                                                          aaaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTCTTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAcc  >  2:345982/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                      CCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACC  >  NZ_CP009273/4091019‑4091278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑