Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,113,018	G→A	R84C (CGC→TGC)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,113,018	0	G	A	100.0%	71.6 / NA	20	R84C (CGC→TGC)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

TGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGC  >  NZ_CP009273/4112909‑4113144                                                                                                              |                                                                                                                               tGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTc                                                                                                   >  1:220396/1‑139 (MQ=255)              gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGt                                                                                         <  2:98811/136‑1 (MQ=255)              gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGt                                                                                         <  2:310482/136‑1 (MQ=255)                   aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAg                                                                                                                            <  2:571632/96‑1 (MQ=255)                   aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAg                                                                                                                            >  1:571632/1‑96 (MQ=255)                   aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCt                                                                                 >  1:448893/1‑139 (MQ=255)                                         caccacCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGAct                                                            <  2:220396/138‑1 (MQ=255)                                                 gCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCttctttct                                                   >  1:109962/1‑139 (MQ=255)                                                     caGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgt                                                >  2:540738/2‑138 (MQ=255)                                                               gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAAt                                      >  1:190596/1‑138 (MQ=255)                                                               gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAAt                                      <  2:190596/138‑1 (MQ=255)                                                                ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                                     <  2:218716/138‑1 (MQ=255)                                                                  cgcacgcaCTCCCGGCTGGCGACTTTCCAGCTGTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg                                                                >  1:560937/1‑109 (MQ=255)                                                                  cgcacgcaCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg                                                                <  2:560937/109‑1 (MQ=255)                                                                  cgcacgcaCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                          >  2:231208/1‑131 (MQ=255)                                                                  cgcacgcaCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                          <  1:231208/131‑1 (MQ=255)                                                                        cacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa                            >  1:268888/1‑139 (MQ=255)                                                                                   ggCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGttt                                                                                                              >  2:282360/1‑46 (MQ=255)                                                                                   ggCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGttt                                                                                                              <  1:282360/46‑1 (MQ=255)                                                                                                     tAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc  <  2:435430/136‑1 (MQ=255)                                                                                                              |                                                                                                                               TGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGC  >  NZ_CP009273/4112909‑4113144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑