Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,192,005	A→G	E51G (GAA→GGA)	zraS →	two‑component system sensor histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,192,005	0	A	G	100.0%	133.5 / NA	34	E51G (GAA→GGA)	zraS	two‑component system sensor histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

TAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGT  >  NZ_CP009273/4191874‑4192124                                                                                                                                    |                                                                                                                        tAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAgg                                                                                                                   >  1:485117/1‑138 (MQ=255)   aaGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTa                                                                                                                 <  1:97393/138‑1 (MQ=255)   aaGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTa                                                                                                                 <  2:600582/138‑1 (MQ=255)        tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTa                                                                                                                 <  1:222277/133‑1 (MQ=255)        tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTa                                                                                                                 >  2:222277/1‑133 (MQ=255)                  aaTGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcgcg                                                                                                  <  2:24067/138‑1 (MQ=255)                                aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCTGCGCTCTGGAGTCggg                                                                                     >  1:269532/1‑137 (MQ=255)                                aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTc                                                                                        >  2:535882/1‑134 (MQ=255)                                aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTc                                                                                        <  1:535882/134‑1 (MQ=255)                                aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCggg                                                                                     >  1:224492/1‑137 (MQ=255)                                aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCggg                                                                                     >  1:41857/1‑137 (MQ=255)                                 tcctccCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                  <  1:479748/139‑1 (MQ=255)                                      cccGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCcgcg                                                                             >  1:602772/1‑139 (MQ=255)                                       ccGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGt                                                                            <  2:41857/139‑1 (MQ=255)                                           ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAggg                                                                        >  2:62047/1‑139 (MQ=255)                                                ttgttgGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                  >  1:392385/1‑124 (MQ=255)                                                ttgttgGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                  <  2:392385/124‑1 (MQ=255)                                                   ttgGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgggatggggat                                                                >  1:286929/1‑139 (MQ=255)                                                      ggCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                  >  2:71130/1‑118 (MQ=255)                                                      ggCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                  <  1:71130/118‑1 (MQ=255)                                                           gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATg                                                         >  1:327353/1‑138 (MQ=255)                                                           gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATg                                                         >  2:47252/1‑138 (MQ=255)                                                            tggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                       <  2:224492/139‑1 (MQ=255)                                                            tggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                       <  2:602772/139‑1 (MQ=255)                                                              ggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                     <  2:231967/139‑1 (MQ=255)                                                               gATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGTCGCGTAGGGATGGGGATGCGAATGCACCa                                                    <  1:267307/139‑1 (MQ=255)                                                               gATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCa                                                    <  1:47252/139‑1 (MQ=255)                                                                      tGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAg                                             >  1:228579/1‑139 (MQ=255)                                                                                              tGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATg                     >  1:262186/1‑139 (MQ=255)                                                                                                   gggcAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCggg                <  1:255721/139‑1 (MQ=255)                                                                                                               cAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAgt    >  2:465575/1‑139 (MQ=255)                                                                                                                 gACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAgtgt  >  1:94563/1‑139 (MQ=255)                                                                                                                              tACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                     <  2:325487/75‑1 (MQ=255)                                                                                                                              tACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                     >  1:325487/1‑75 (MQ=255)                                                                                                                                    |                                                                                                                        TAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGT  >  NZ_CP009273/4191874‑4192124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑