Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,245,208	T→C	D396G (GAT→GGT)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,245,208	0	T	C	100.0%	167.4 / NA	46	D396G (GAT→GGT)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

GTTCTTTGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGATCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAA  >  NZ_CP009273/4245080‑4245340                                                                                                                                 |                                                                                                                                     gTTCTTTGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGAACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACg                                                                                                                            <  1:283949/139‑1 (MQ=255)    cTTTGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          <  2:324240/138‑1 (MQ=255)    cTTTGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          <  1:5248/138‑1 (MQ=255)       tGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCg                                                                                                                       >  1:480599/1‑138 (MQ=255)            tAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACgg                                                                                                                  >  2:332867/1‑138 (MQ=255)                      cTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGcc                                                                                                       <  2:480599/139‑1 (MQ=255)                         ccATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACg                                                                                                                            <  2:405337/115‑1 (MQ=255)                         ccATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACg                                                                                                                            >  1:405337/1‑115 (MQ=255)                            tGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCAc                                                                                                 >  2:64897/1‑139 (MQ=255)                              aCGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACg                                                                                                <  2:207137/138‑1 (MQ=255)                                      aTAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCg                                                                                       <  1:539440/139‑1 (MQ=255)                                             atatAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGaa                                                                                 >  2:175777/1‑138 (MQ=255)                                                taGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAAc                                                                             >  1:397331/1‑139 (MQ=255)                                                taGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAAc                                                                             <  2:397331/139‑1 (MQ=255)                                                  gATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          >  1:370472/1‑92 (MQ=255)                                                  gATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          <  2:370472/92‑1 (MQ=255)                                                       gAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCt                                                                      >  1:119237/1‑139 (MQ=255)                                                        aaTCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTg                                                                     >  2:430271/1‑139 (MQ=255)                                                               gTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGtt                                                              <  2:532999/139‑1 (MQ=255)                                                                  atcctaCGCGTGCCGCCACGCAGCATCGCCTTAATGGTCAGCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGc                                                           <  1:510187/134‑1 (MQ=255)                                                                  aTCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGc                                                           >  1:5159/1‑139 (MQ=255)                                                                       aCGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCgaga                                                      <  2:400365/139‑1 (MQ=255)                                                                        cgcgTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCgaga                                                      >  2:70189/1‑138 (MQ=255)                                                                                 ccACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCgg                                             >  2:227053/1‑138 (MQ=255)                                                                                       agcaTCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACgg                                      <  1:70189/139‑1 (MQ=255)                                                                                              ccTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          >  2:390657/1‑48 (MQ=255)                                                                                              ccTGAATGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGcc                                                                                                                          <  1:390657/48‑1 (MQ=255)                                                                                                   aTGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGa                                                                                  >  1:135385/1‑83 (MQ=255)                                                                                                   aTGGTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGa                                                                                  <  2:135385/83‑1 (MQ=255)                                                                                                      gTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTa                        <  2:380071/138‑1 (MQ=255)                                                                                                      gTCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTAt                       <  1:376898/139‑1 (MQ=255)                                                                                                       tCATCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTAtt                      <  2:5159/139‑1 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACg                                                                                                                            >  1:197822/1‑35 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACg                                                                                                                            <  2:197822/35‑1 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGACTAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGc                    >  1:288889/1‑139 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGc                    >  1:8826/1‑139 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGc                    >  1:78188/1‑139 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGc                    >  2:529726/1‑139 (MQ=255)                                                                                                         aTCGACAGCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGc                    >  2:233085/1‑139 (MQ=255)                                                                                                                gCGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTaaa             >  2:319262/1‑139 (MQ=255)                                                                                                                 cGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCg                                                            <  2:459244/91‑1 (MQ=255)                                                                                                                 cGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCg                                                            >  1:459244/1‑91 (MQ=255)                                                                                                                 cGTACCAGTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAAc            >  2:263298/1‑139 (MQ=255)                                                                                                                        gTTTTCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGc     <  1:529726/139‑1 (MQ=255)                                                                                                                         ttttCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGc     >  2:358389/1‑138 (MQ=255)                                                                                                                           ttCGGACCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGaa  >  2:295641/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     GTTCTTTGGCGTAAGTACCCACTTCCATGACGTGCTCATAACCGATATAGATCGGAATCAGCGTAATCGGACGCGTGCCGCCACGCAGCATCGCCTGAATGGTCATCGACAGCGTACCAGTTTTCGGATCCAGCAAACGCCCCGTACGGGAACGACCGCCTTCCACGAAGTACTCGACGGAATAACCACGGCTGAACAGTTCGCCGAGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAA  >  NZ_CP009273/4245080‑4245340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑