Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,320,763	T→C	S149P (TCG→CCG)	proP →	glycine betaine/L‑proline transporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,320,763	0	T	C	100.0%	185.9 / NA	52	S149P (TCG→CCG)	proP	glycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/20);  total (32/20)

ATTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGGCGAAGC  >  NZ_CP009273/4320631‑4320896                                                                                                                                     |                                                                                                                                      aTTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGCGAATATACCGGGGCGCCGatat                                                                                                                                 >  2:28746/1‑139 (MQ=255)    aTGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                                              >  2:116343/1‑139 (MQ=255)       tCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                           <  2:27433/139‑1 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCg                                                                                                                         <  2:568686/139‑1 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCg                                                                                                                         >  2:282384/1‑139 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGCTGCg                                                                                                                         <  2:121742/139‑1 (MQ=255)              gTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                                                    <  1:298949/139‑1 (MQ=255)                      tGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                                                             >  1:68922/1‑138 (MQ=255)                        tATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGAc                                                                                                          >  1:326810/1‑139 (MQ=255)                         aTTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgtt                                                                                                                            >  2:285385/1‑120 (MQ=255)                         aTTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgtt                                                                                                                            <  1:285385/120‑1 (MQ=255)                          ttGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGAcc                                                                                                         <  1:487723/138‑1 (MQ=255)                           tGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                                                    <  1:317155/126‑1 (MQ=255)                           tGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                                                    >  2:317155/1‑126 (MQ=255)                                tAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACg                                                                                                  >  1:531439/1‑139 (MQ=255)                                  aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGACTACTCCACTGACCGTAAACGTg                                                                                                >  2:124578/1‑139 (MQ=255)                                  aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTg                                                                                                >  2:592524/1‑139 (MQ=255)                                  aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTg                                                                                                >  1:84747/1‑139 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                                                    >  2:253679/1‑114 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                                                    <  1:253679/114‑1 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                                                             <  1:86618/121‑1 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                                                             >  2:86618/1‑121 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCtt                                                                                            >  2:180384/1‑138 (MQ=255)                                        tCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTa                                                                                          >  2:331064/1‑139 (MQ=255)                                        tCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTa                                                                                          >  1:416419/1‑139 (MQ=255)                                        tCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTa                                                                                          >  2:636947/1‑139 (MQ=255)                                          cTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTAt                                                                                         <  1:116343/138‑1 (MQ=255)                                             cGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATggg                                                                                      >  2:119258/1‑138 (MQ=255)                                              gACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCa                                                                                    <  2:326810/139‑1 (MQ=255)                                                         tATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGAc                                                                         <  2:68922/139‑1 (MQ=255)                                                                gCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGtt                                                                  <  2:535332/139‑1 (MQ=255)                                                                  aCCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTc                                                                 >  1:138441/1‑138 (MQ=255)                                                                   ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGt                                                                                                 <  1:378741/105‑1 (MQ=255)                                                                   ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGt                                                                                                 >  2:378741/1‑105 (MQ=255)                                                                                 gATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTtgtg                                                 >  2:103505/1‑139 (MQ=255)                                                                                       tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               <  1:592524/135‑1 (MQ=255)                                                                                       tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                                               <  1:331064/135‑1 (MQ=255)                                                                                         aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                           <  1:555995/57‑1 (MQ=255)                                                                                         aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                           >  2:555995/1‑57 (MQ=255)                                                                                         aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGc                                         <  1:103505/139‑1 (MQ=255)                                                                                            tGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCggg                                      >  1:26128/1‑139 (MQ=255)                                                                                            tGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCggg                                      >  2:211256/1‑139 (MQ=255)                                                                                              gCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCg                                    <  1:362275/139‑1 (MQ=255)                                                                                               cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                           >  2:444614/1‑51 (MQ=255)                                                                                               cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttgttg                                                                                                                           <  1:444614/51‑1 (MQ=255)                                                                                               cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGc                                     >  2:362275/1‑137 (MQ=255)                                                                                                              tcggCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTc                    >  2:520835/1‑139 (MQ=255)                                                                                                                            aCCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgc       >  1:592829/1‑138 (MQ=255)                                                                                                                            aCCGGGGCGCCGATATTTGTTGCGGAATAACACCCTGACCGTAAACGGGGCTTTATGGGGAGCAGGCTGGAATTCGGTTCTATTGACGGGTTCGTGCTGGGAGCGGGCGTGGAGGTGTTAATTTCGAACATTGTCgggg      >  2:245124/1‑137 (MQ=255)                                                                                                                                 ggCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaagc  >  1:38339/1‑138 (MQ=255)                                                                                                                                 ggCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaagc  >  1:395494/1‑138 (MQ=255)                                                                                                                                 ggCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGgcgaagc  >  1:639365/1‑138 (MQ=255)                                                                                                                                     |                                                                                                                                      ATTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGTGGTGTTAATTTCGACCATTGTCGGCGAAGC  >  NZ_CP009273/4320631‑4320896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑