Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,389,340	A→G	E91G (GAA→GGA)	miaA →	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,389,340	0	A	G	100.0%	82.9 / NA	24	E91G (GAA→GGA)	miaA	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/15);  total (9/15)

TTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACA  >  NZ_CP009273/4389205‑4389475                                                                                                                                        |                                                                                                                                        tttACAAAGGGGTGGAGATCGGGGCGGCGAAGCCGAACGCTGAAGAGGTACGCGCCGCGCCGCACCGAGTGCTGGATAGTCGGGATCCGTCGCAGGCTTACGCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGgaa                                                                                                                                       <  2:72067/138‑1 (MQ=255)   tACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATg                                                                                                                                     <  1:29243/138‑1 (MQ=255)     cAAAGGGATGGATATCGTGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCTCCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGc                                                                                                                                   <  2:525297/138‑1 (MQ=255)       aaGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGa                                                                                                                                >  1:298000/1‑139 (MQ=255)                 tatCGGGACGGCGAAGCCGAACGCGGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACcgcg                                                                                                                      <  1:311317/139‑1 (MQ=255)                    cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCgcggcg                                                                                                                   <  2:187657/139‑1 (MQ=255)                        aCGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCggcggggc                                                                                                               <  1:447608/139‑1 (MQ=255)                                           aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            <  2:461674/139‑1 (MQ=255)                                           aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            >  2:398902/1‑139 (MQ=255)                                           aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                                            >  1:466396/1‑139 (MQ=255)                                                   cTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGa                                                                                    >  2:566960/1‑139 (MQ=255)                                                        cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               <  1:398902/139‑1 (MQ=255)                                                        cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                                               <  2:161739/139‑1 (MQ=255)                                                          cgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGCTGCGCTGGCGGGAAAGGCCGGTATTACCGCGGCGGGGCGGCTTCCACCGTTTGTGGGCGGGACGGTGTTGTa                                                                             >  2:354400/1‑139 (MQ=255)                                                                                     aTCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTc                                                  >  1:576022/1‑139 (MQ=255)                                                                                         gTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTcgccg                                              <  1:124421/139‑1 (MQ=255)                                                                                                    aCTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGGTCCCACTGTTAGTGGGGGGGACGATGTTGTATTTCAAGGCATTGCTGGGAGGGTTGTCGGCGCTCACGTCgg                                    >  2:306832/1‑138 (MQ=255)                                                                                                          ctgctgATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGAccc                             >  1:604807/1‑139 (MQ=255)                                                                                                          ctgctgATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGAccc                             >  1:36053/1‑139 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa                      <  2:466396/139‑1 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa                      <  1:306832/139‑1 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa                      <  2:178336/139‑1 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa                      <  1:371315/139‑1 (MQ=255)                                                                                                                                gcTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGTGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAg       <  1:74914/139‑1 (MQ=255)                                                                                                                                     cGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACa  <  1:566960/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACA  >  NZ_CP009273/4389205‑4389475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑