Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,401,268	T→G	G50G (GGT→GGG)	yjfK →	YjfK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,401,268	0	T	G	100.0%	104.8 / NA	29	G50G (GGT→GGG)	yjfK	YjfK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/13);  total (16/13)

GCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGA  >  NZ_CP009273/4401147‑4401393                                                                                                                          |                                                                                                                              gCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                              <  2:112303/139‑1 (MQ=255)       ataataAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGGAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgccg                                                                                                            >  1:32727/1‑135 (MQ=255)       ataataAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgccg                                                                                                            <  2:32727/135‑1 (MQ=255)                cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACAt                                                                                               >  2:435654/1‑139 (MQ=255)                cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACAt                                                                                               >  2:310299/1‑139 (MQ=255)                        gCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTgg                                                                                       >  2:437658/1‑139 (MQ=255)                             tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGggcggc                                                                                  >  2:532127/1‑139 (MQ=255)                               gTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGcggcgg                                                                                <  2:50871/139‑1 (MQ=255)                                          cTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                     >  2:482904/1‑139 (MQ=255)                                          cTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                     >  2:467428/1‑139 (MQ=255)                                             cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                                                                   <  1:572574/138‑1 (MQ=255)                                             cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                                                                   <  2:152027/138‑1 (MQ=255)                                                   aaTAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                                                                <  1:131676/135‑1 (MQ=255)                                                   aaTAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGc                                                                <  2:339186/135‑1 (MQ=255)                                                          gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                            <  2:294813/68‑1 (MQ=255)                                                          gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                            >  1:294813/1‑68 (MQ=255)                                                          gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt                                                      <  1:310299/138‑1 (MQ=255)                                                          gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt                                                      >  2:618089/1‑138 (MQ=255)                                                                     gACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGt                                          >  1:70708/1‑139 (MQ=255)                                                                               cgtttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAAt                                >  2:100009/1‑139 (MQ=255)                                                                                 tttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTg                                     >  2:509450/1‑132 (MQ=255)                                                                                 tttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTg                                     <  1:509450/132‑1 (MQ=255)                                                                                    cgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCcaca                                                                                                <  1:244670/70‑1 (MQ=255)                                                                                    cgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCcaca                                                                                                >  2:244670/1‑70 (MQ=255)                                                                                            ggAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCATCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACcggc                   >  2:9125/1‑139 (MQ=255)                                                                                                            aTTGCGCTGCGGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgat    <  2:102645/138‑1 (MQ=255)                                                                                                            aTTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatg   <  1:482904/139‑1 (MQ=255)                                                                                                              tGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatga  >  2:607425/1‑138 (MQ=255)                                                                                                              tGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatga  >  1:296042/1‑138 (MQ=255)                                                                                                                          |                                                                                                                              GCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGA  >  NZ_CP009273/4401147‑4401393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑