Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,498,429	T→G	R2S (AGA→AGC)	BW25113_RS22240 ←	IS30‑like element IS30 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,498,429	0	T	G	100.0%	40.1 / NA	13	R2S (AGA→AGC)	BW25113_RS22240	IS30‑like element IS30 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/7);  total (6/7)

TTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTT  >  NZ_CP009273/4498374‑4498563                                                        |                                                                                                                                       ttCCGTTCTTCCATATTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAat                                                      <  1:303083/138‑1 (MQ=21)    cGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAatatat                                                  <  2:558016/139‑1 (MQ=37)           ccATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCt                                            >  2:311392/1‑138 (MQ=255)                    aaaaaCAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATG‑TTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCt                                 <  2:158082/139‑1 (MQ=255)                           gagGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAg                                   >  1:365273/1‑131 (MQ=255)                           gagGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAg                                   <  2:365273/131‑1 (MQ=255)                            agGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTa                          <  2:457162/139‑1 (MQ=255)                            agGCTTTTTCATCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTa                          <  2:469493/139‑1 (MQ=255)                                      ctctGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACg                <  1:293898/139‑1 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  1:34063/1‑138 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  1:391842/1‑138 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  2:563167/1‑138 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATCTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt  >  1:559619/1‑138 (MQ=255)                                                        |                                                                                                                                       TTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTT  >  NZ_CP009273/4498374‑4498563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑