| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,507,805 | T→C | E83G (GAA→GGA) | fecI ← | RNA polymerase sigma factor FecI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,507,805 | 0 | T | C | 100.0% | 119.1 / NA | 32 | E83G (GAA→GGA) | fecI | RNA polymerase sigma factor FecI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/17); total (15/17) | |||||||||||
CGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCA > NZ_CP009273/4507686‑4507930 | cGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGaaa < 1:379769/139‑1 (MQ=255) gTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAAc < 2:292130/139‑1 (MQ=255) gTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTccac > 2:345787/1‑138 (MQ=255) cATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAcg < 2:634991/139‑1 (MQ=255) cATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAcg < 1:345787/139‑1 (MQ=255) cATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCCCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATCCGCTTTTCCCAGCGCTTTTCGGCGAAACAGGTCCACCATCAcg < 1:550304/139‑1 (MQ=255) gTAGGGTTTCGAGTTGGCTTTCGCGTTCCCCAGGTGAAGGCGTTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGc < 1:479794/138‑1 (MQ=255) gTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTg > 1:519413/1‑137 (MQ=255) tctcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGagg > 1:257896/1‑137 (MQ=255) tcGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGaggaag > 2:281829/1‑138 (MQ=255) gcgTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCgg < 2:257896/139‑1 (MQ=255) gAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa > 1:407240/1‑97 (MQ=255) gAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa < 2:407240/97‑1 (MQ=255) ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGttt > 1:504417/1‑139 (MQ=255) ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGttt > 2:417459/1‑139 (MQ=255) cTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCt < 2:504417/139‑1 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa < 1:448711/89‑1 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGa > 2:448711/1‑89 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGTAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCt > 1:203629/1‑138 (MQ=255) tCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTa > 1:477531/1‑139 (MQ=255) cATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCg > 1:297326/1‑138 (MQ=255) cATAAGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCg > 1:289024/1‑138 (MQ=255) aGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCgg > 1:577707/1‑42 (MQ=255) aGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCgg < 1:553244/42‑1 (MQ=255) aGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCgg > 2:553244/1‑42 (MQ=255) aGCGCCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCgg < 2:577707/42‑1 (MQ=255) cgcCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaaa < 2:303763/139‑1 (MQ=255) cgcCAGCATCTCCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaaa < 1:476050/139‑1 (MQ=255) tcCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCg > 2:414925/1‑108 (MQ=255) tcCAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCg < 1:414925/108‑1 (MQ=255) cAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCa < 2:289024/139‑1 (MQ=255) cAGATACGCTTTTCCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCa < 2:558064/139‑1 (MQ=255) | CGTTTAGCCCGTCCAGCATGCTGTCGAGGAGTTGTAGGGTCTCGAGTTGGCTTTCGCGTTCCTCAGGTGAAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCA > NZ_CP009273/4507686‑4507930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |