Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	294,817	A→T	V610E (GTG→GAG)	paoC ←	aldehyde oxidoreductase molybdenum‑binding subunit PaoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	294,817	0	A	T	100.0%	95.5 / NA	27	V610E (GTG→GAG)	paoC	aldehyde oxidoreductase molybdenum‑binding subunit PaoC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/16);  total (11/16)

TTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACACAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTC  >  NZ_CP009273/294688‑294952                                                                                                                                  |                                                                                                                                        ttAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCa                                                                                                                                <  2:149013/139‑1 (MQ=255)            gCCCAAACGGTCCTCCCCCGCCCGCTCCTCCCTCCGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCCGGATGCGTCCTGCAGCCCCCTCCGCGAGCCTTCGCCGGAcc                                                                                                                     <  2:614964/139‑1 (MQ=255)                         tCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCg                                                                                                        <  1:257515/139‑1 (MQ=255)                              cGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGGcgcg                                                                                                   <  2:442416/139‑1 (MQ=255)                                ccAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCt                                                                                                 <  2:176463/139‑1 (MQ=255)                                ccAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGCATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCgcgc                                                                                                  >  1:405275/1‑138 (MQ=255)                                            tCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGAc                                                                                     <  1:325082/139‑1 (MQ=255)                                              aGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCt                                                                                   <  1:517492/139‑1 (MQ=255)                                                 gCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCca                                                                                >  1:510976/1‑139 (MQ=255)                                                     cgcCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACaa                                                                             >  1:24574/1‑138 (MQ=255)                                                         catgcccatAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGc                                                                        <  2:24574/139‑1 (MQ=255)                                                          atgcccatAGTCATTGCGCCAAGGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGcc                                                                       <  2:529432/139‑1 (MQ=255)                                                                  aGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAACATGCCCGGCACAg                                                               >  2:440539/1‑139 (MQ=255)                                                                  aGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAg                                                               >  1:88012/1‑139 (MQ=255)                                                                      aTTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTc                                                            >  1:146245/1‑138 (MQ=255)                                                                       ttGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGa                                                          >  2:306988/1‑139 (MQ=255)                                                                        tGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGAc                                                         >  2:279489/1‑139 (MQ=255)                                                                          cgcCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGActg                                                       <  1:636076/139‑1 (MQ=255)                                                                          cgcCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGAct                                                        <  1:279489/138‑1 (MQ=255)                                                                            ccAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGAc                                                         >  1:46046/1‑135 (MQ=255)                                                                            ccAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCGGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGAc                                                         <  2:46046/135‑1 (MQ=255)                                                                               aTGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGt                                                  <  2:146245/139‑1 (MQ=255)                                                                                  aCCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTAct                                               <  1:594420/139‑1 (MQ=255)                                                                                    cTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTActct                                             <  1:434860/139‑1 (MQ=255)                                                                                            gcgcAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAg                                     >  2:548657/1‑139 (MQ=255)                                                                                                 gTTTTCGGATTCAGGATGCGTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTc                                >  2:293266/1‑139 (MQ=255)                                                                                                                    gTCCTGCAGCACACTCAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTc             <  2:88012/139‑1 (MQ=255)                                                                                                                               cactcaGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTc             >  2:422791/1‑128 (MQ=255)                                                                                                                               cactcaGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTc             <  1:422791/128‑1 (MQ=255)                                                                                                                               cactcaGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTc  >  2:526556/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        TTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGCACACACAGCGAGCATACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTC  >  NZ_CP009273/294688‑294952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑