Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 338,646 A→T M18L (ATG→TTG)  yahK → NADPH‑dependent aldehyde reductase YahK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273338,6460AT100.0% 128.5 / NA 33M18L (ATG→TTG) yahKNADPH‑dependent aldehyde reductase YahK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/16);  total (17/16)

GGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGC  >  NZ_CP009273/338514‑338772
                                                                                                                                    |                                                                                                                              
ggCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGata                                                                                                                          >  1:334734/1‑139 (MQ=255)
            tGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGt                                                                                                               >  2:356567/1‑138 (MQ=255)
                                          gCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaaa                                                                                <  1:356567/139‑1 (MQ=255)
                                             ttGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAatga                                                                                              <  2:567161/122‑1 (MQ=255)
                                             ttGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAatga                                                                                              >  1:567161/1‑122 (MQ=255)
                                               gCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCt                                                                           >  2:409301/1‑139 (MQ=255)
                                               gCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCt                                                                           >  2:211821/1‑139 (MQ=255)
                                                          aTAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGt                                                                >  1:143356/1‑139 (MQ=255)
                                                          aTAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGt                                                                >  1:484317/1‑139 (MQ=255)
                                                             gCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTg                                                             <  2:324499/139‑1 (MQ=255)
                                                                     ggAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCg                                                     <  2:484317/139‑1 (MQ=255)
                                                                        gTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                                  >  1:323168/1‑139 (MQ=255)
                                                                        gTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                                  <  1:349503/139‑1 (MQ=255)
                                                                         tAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtct                                                 <  1:605639/139‑1 (MQ=255)
                                                                           aaCACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtctc                                                >  1:195415/1‑138 (MQ=255)
                                                                           aaCACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtctc                                                >  2:365866/1‑138 (MQ=255)
                                                                           aaCACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtctc                                                >  2:175444/1‑138 (MQ=255)
                                                                                            aGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAg                              <  2:325440/139‑1 (MQ=255)
                                                                                                 ttGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGc                         >  2:480469/1‑139 (MQ=255)
                                                                                                       cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                    <  1:68530/138‑1 (MQ=255)
                                                                                                       cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                    <  2:581379/138‑1 (MQ=255)
                                                                                                       cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                    <  1:247955/138‑1 (MQ=255)
                                                                                                       cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                    <  1:365866/138‑1 (MQ=255)
                                                                                                       cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa                    <  2:334734/138‑1 (MQ=255)
                                                                                                           ttCCGCTAAACAACCACTTGAGCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGtt               <  2:400843/139‑1 (MQ=255)
                                                                                                               gCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTAcc           >  2:296328/1‑139 (MQ=255)
                                                                                                                        ccACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGc  >  1:565579/1‑139 (MQ=255)
                                                                                                                             tGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACt                                                                       >  1:531389/1‑65 (MQ=255)
                                                                                                                             tGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACt                                                                       <  2:531389/65‑1 (MQ=255)
                                                                                                                               aaCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCt                                                                           >  2:356767/1‑59 (MQ=255)
                                                                                                                               aaCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCt                                                                           <  1:356767/59‑1 (MQ=255)
                                                                                                                               aaCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                             >  1:415653/1‑89 (MQ=255)
                                                                                                                               aaCCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                                             <  2:415653/89‑1 (MQ=255)
                                                                                                                                    |                                                                                                                              
GGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGC  >  NZ_CP009273/338514‑338772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑