Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	351,491	T→G	S287A (TCG→GCG)	codB →	cytosine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	351,491	0	T	G	100.0%	90.7 / NA	26	S287A (TCG→GCG)	codB	cytosine permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/13);  total (13/13)

CGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGACCTTCCTTTCGGCAG  >  NZ_CP009273/351370‑351623                                                                                                                          |                                                                                                                                     cgcTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGcc                                                                                                                     <  1:279027/139‑1 (MQ=255) cgcTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGcc                                                                                                                     <  2:293094/139‑1 (MQ=255)              ggCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGc                                                                                                                      >  2:502376/1‑125 (MQ=255)              ggCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGc                                                                                                                      <  1:502376/125‑1 (MQ=255)                                    tgatgaTTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCgg                                                                                   <  2:396895/138‑1 (MQ=255)                                      atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCCACATTACCGGGTTGTCGAGCAAAACCCTTTCCGTaa                                                                                >  2:530125/1‑139 (MQ=255)                                      atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGGTGTCGAGCAAAACCCTTTCGGTaa                                                                                >  1:459791/1‑139 (MQ=255)                                      atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTaa                                                                                >  2:31830/1‑139 (MQ=255)                                      atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTAACGGGGTGTCGGGCGAAACCCTTTCAGtga                                                                                >  2:389628/1‑137 (MQ=255)                                             cTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACg                                                                          <  2:358673/138‑1 (MQ=255)                                             cTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTGCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACg                                                                          <  1:389628/138‑1 (MQ=255)                                                  ggCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGtatta                                                                    <  1:511373/139‑1 (MQ=255)                                                  ggCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGtatta                                                                    >  1:36200/1‑139 (MQ=255)                                                       gctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCgg                                                                >  1:489928/1‑138 (MQ=255)                                                        ctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGt                                                               <  1:31830/138‑1 (MQ=255)                                                        ctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGt                                                               <  2:479378/138‑1 (MQ=255)                                                            tgcctgcGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGt                                                          >  1:516611/1‑139 (MQ=255)                                                               ctgcGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTg                                                       <  1:133715/139‑1 (MQ=255)                                                                        tggtgCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTAt                                               <  2:36200/138‑1 (MQ=255)                                                                          gtgCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATgg                                             <  1:531691/138‑1 (MQ=255)                                                                                     gAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTTGCCAACATTACCGGGATGTCGAGCCAAACCCTTTCGGGAATCAACGGTTTTTTCGGTAAGGTCTGCGCATTATGGCTGTATAACAAt                                 >  1:480117/1‑139 (MQ=255)                                                                                          tCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCg                                                                                    >  1:328646/1‑83 (MQ=255)                                                                                          tCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCg                                                                                    <  2:328646/83‑1 (MQ=255)                                                                                              gACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGc                        >  1:636866/1‑139 (MQ=255)                                                                                                                   ctATGCGGCGGGTTTAAGTTTCGCCAACATTACCGGGGTGGCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGGATAACAATTTTGTCGGCTGGGTGGCCTTCCTTTCgggc   >  2:64771/1‑137 (MQ=255)                                                                                                                    tATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGGAATCAACGGTATTATCGGGACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGACCTTCCTTTCGGCAg  >  1:92193/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                     CGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGACCTTCCTTTCGGCAG  >  NZ_CP009273/351370‑351623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑