Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	393,907	A→G	K194E (AAA→GAA)	yaiW →	surface‑exposed outer membrane lipoprotein YaiW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	393,907	0	A	G	100.0%	133.8 / NA	37	K194E (AAA→GAA)	yaiW	surface‑exposed outer membrane lipoprotein YaiW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/15);  total (22/15)

TGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGC  >  NZ_CP009273/393800‑394034                                                                                                            |                                                                                                                                tGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCtt                                                                                                  >  2:564624/1‑139 (MQ=255) tGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCtt                                                                                                  >  2:264321/1‑139 (MQ=255) tGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCtt                                                                                                  >  1:222475/1‑139 (MQ=255)      aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTc                                                                                             >  2:21626/1‑139 (MQ=255)      aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTc                                                                                             >  1:566961/1‑139 (MQ=255)           gCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTcgccgc                                                                                        >  2:576271/1‑139 (MQ=255)                     tttGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACa                                                                                                                   >  1:188829/1‑102 (MQ=255)                     tttGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACa                                                                                                                   <  2:188829/102‑1 (MQ=255)                     tttGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTtg                                                                               <  1:275203/138‑1 (MQ=255)                             gCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGGTGTGGTTTgg                                                                       >  2:208853/1‑138 (MQ=255)                                         gcgcACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATgg                                                                                                                           >  1:382345/1‑74 (MQ=255)                                         gcgcACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATgg                                                                                                                           <  2:382345/74‑1 (MQ=255)                                                 gTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGGTGTGGTTTGGTACTTACCATTTACTGAATTa                                                  >  2:233132/1‑139 (MQ=255)                                                       cGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            <  1:576271/139‑1 (MQ=255)                                                       cGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            <  1:275097/139‑1 (MQ=255)                                                         aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCg                                             <  1:590983/136‑1 (MQ=255)                                                         aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCg                                             >  2:590983/1‑136 (MQ=255)                                                         aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  1:135072/1‑137 (MQ=255)                                                         aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  2:473898/1‑137 (MQ=255)                                                         aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  1:220948/1‑137 (MQ=255)                                                                  aGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                 <  1:21626/139‑1 (MQ=255)                                                                  aGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                 <  1:51737/139‑1 (MQ=255)                                                                    cATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCgcggcg                                                                                    >  2:42332/1‑86 (MQ=255)                                                                    cATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCgcggcg                                                                                    <  1:42332/86‑1 (MQ=255)                                                                    cATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCa                               >  2:23485/1‑139 (MQ=255)                                                                         cTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGa                                                      <  1:284966/111‑1 (MQ=255)                                                                         cTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGa                                                      >  2:284966/1‑111 (MQ=255)                                                                            ttGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGata                         <  2:487740/137‑1 (MQ=255)                                                                             tGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGAtata                       <  2:443430/138‑1 (MQ=255)                                                                                      gCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCt             <  1:23485/139‑1 (MQ=255)                                                                                                gggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACg   <  1:544318/139‑1 (MQ=255)                                                                                                 ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgc  >  2:219542/1‑139 (MQ=255)                                                                                                 ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgc  >  2:417613/1‑139 (MQ=255)                                                                                                 ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgc  >  1:261226/1‑139 (MQ=255)                                                                                                 ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTCCTGATTTTAACgc  >  2:208049/1‑139 (MQ=255)                                                                                                     tCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                 >  2:412431/1‑104 (MQ=255)                                                                                                     tCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                 <  1:412431/104‑1 (MQ=255)                                                                                                            |                                                                                                                                TGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGC  >  NZ_CP009273/393800‑394034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑