Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 415,362 T→C S106P (TCC→CCC)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273415,3620TC100.0% 81.1 / NA 24S106P (TCC→CCC) brnQbranched‑chain amino acid transporter carrier protein BrnQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/13);  total (11/13)

GTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGCTGCTG  >  NZ_CP009273/415227‑415478
                                                                                                                                       |                                                                                                                    
gTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTccc                                                                                                                    <  1:367484/138‑1 (MQ=255)
 tGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCtt                                                                                                                  >  1:89216/1‑139 (MQ=255)
        ggCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTg                                                                                                           >  2:466698/1‑139 (MQ=255)
        ggCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTg                                                                                                           >  2:287951/1‑139 (MQ=255)
        ggCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTg                                                                                                           >  1:295324/1‑139 (MQ=255)
                  ggcggcGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTgcg                                                                                                  <  1:459698/138‑1 (MQ=255)
                                   ccTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTcc                                                                                >  1:576488/1‑139 (MQ=255)
                                    cTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCcg                                                                               >  1:517159/1‑139 (MQ=255)
                                       aGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCcgcg                                                                             <  2:535538/138‑1 (MQ=255)
                                       aGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCcgcg                                                                             <  2:576488/138‑1 (MQ=255)
                                             ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                                                                      <  2:295324/139‑1 (MQ=255)
                                                   ggTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGttt                                                                 <  2:250519/138‑1 (MQ=255)
                                                       aaGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTa                                                            <  2:123301/139‑1 (MQ=255)
                                                            gCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGcc                                                       <  2:523710/139‑1 (MQ=255)
                                                                          ggCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGc                                          >  2:166565/1‑138 (MQ=255)
                                                                           gCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGAGATTGCGCCGCTGACAGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGc                                          <  1:287951/137‑1 (MQ=255)
                                                                              aCGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCg                                     <  2:175840/139‑1 (MQ=255)
                                                                                                      ccGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCgg             >  1:286178/1‑139 (MQ=255)
                                                                                                       cGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCggg            >  2:183921/1‑139 (MQ=255)
                                                                                                         cTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGc           >  2:250397/1‑138 (MQ=255)
                                                                                                         cTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCa          <  1:183921/139‑1 (MQ=255)
                                                                                                         cTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCa          <  1:166565/139‑1 (MQ=255)
                                                                                                                   cgccgcGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGctgctg  <  1:93892/137‑1 (MQ=255)
                                                                                                                                  ccGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGc                                                        <  1:6985/68‑1 (MQ=255)
                                                                                                                                  ccGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGc                                                        >  2:6985/1‑68 (MQ=255)
                                                                                                                                       |                                                                                                                    
GTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGCTGCTG  >  NZ_CP009273/415227‑415478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑